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FGF-2 species-specific discovery kit (Qk501)

For rapid testing of multiple species FGF-2 and thermostable FGF-2 to replace human proteins in cell cultivated meat.

 

FGF2 is commonly used in cultivated meat cell culture for the differentiation and maintenance of cells, but human FGF-2 is often used for this process.

It is generally accepted that best practice is to use growth factors from the target species for reasons of consumer acceptance, regulatory considerations and species-specific activity. Receptor binding affinity and efficacy may differ depending on each species, resulting in higher concentration necessary for desired results in culture if non-species specific FGF-2 is used.

The FGF-2 species-specific Discovery Kit (Qk501) contains 6 different forms of FGF-2, allowing easy optimization studies for researchers looking to replace human growth factors with species-specific FGF-2, as well as thermostable versions of FGF-2 proteins.

Fast & free worldwide shipping.
Orders are typically shipped same or next day (except Friday).

Buy online with secure credit card or purchase order. You can also place your order by email orders@qkine.com

Kit content

  • Zebrafish FGF2  – Qk002 – 100 µg
    Used extensively to support the maintenance and proliferation of human and mouse induced pluripotent (iPSC) and embryonic stem cells (ESC)

  • Bovine / porcine FGF2 (154 aa) – Qk056 – 50 µg
    The long form of FGF-2 used for the development of optimized serum-free culture media for species-specific bovine (cow) and porcine (pig) cultivated meat and veterinary research applications.

  • Bovine / porcine FGF2 (145 aa) – Qk040 – 50 µg
    For the development of species-specific bovine (cow) and porcine (pig) cellular agriculture protocols and veterinary research applications.

  • Bovine/porcine FGF2-G3 (154 aa) – Qk081 – 50 µg
    A thermostable engineered form of bovine FGF-2. This is the 154 aa mature domain of FGF-2 (Qk056). The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3)

  • Bovine/porcine FGF2-G3 (145 aa) – Qk080 – 50 µg
    thermostable engineered form of bovine FGF-2. This is the 145 aa mature domain of FGF-2 (Qk040). The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3)

  • Human FGF2-G3 (154 aa)  – Qk053 – 50 µg
    A thermostable engineered form of human FGF-2. Qk053 is the 154 aa mature domain of FGF-2 (Qk027). The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3).

Fish FGF-2 coming soon to this kit. Contact us to express your interest.

Quality features

  • Highly pure >98%, by SDS-PAGE quantitative densitometry

  • Highly bioactive – come with our Bioactivity Guarantee

  • Source: Expressed in E. coli 

  • Animal-free (AOF) and carrier protein-free

  • Manufactured in Cambridge, UK

  • Lyophilized

Quality assays

  • Mass spectrometry, single species with the expected mass

  • Endotoxin: <0.005 EU/μg protein (below the level of detection)

  • Recovery from stock vial: >95%

Kit description

Fibroblast growth factor 2 (FGF-2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles, including regulation of cell proliferation and differentiation. FGF-2 is used to support the maintenance of embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. [1-3]

FGF-2 is inherently unstable and prone to proteolytic degradation and aggregation. This fundamental biochemical instability, and therefore low half-life in culture media (<10 h), is an important contribution to the need for frequent media changes and challenges in improving homogeneity during stem cell proliferation and subsequent differentiation. To improve the stability of FGF-2 for stem cell and cultivated meat applications, an optimal set of nine amino acid substitutions that stabilize FGF-2 were identified. The biological activity of wild-type FGF-2 is <50% after 10h incubation with conditioned media. In contrast, no reduction in FGF2-G3 biological activity is observed after >7 days incubation with conditioned media at 37 oC.

It is generally accepted that best practice is to use growth factors from the target species for reasons of consumer acceptance, regulatory considerations and species-specific activity including differences in receptor binding and efficacy in some cases. Some growth factor protein sequences are highly conserved and 100% identical across species. There is currently a lack of commercial availability of cultured-meat and fish relevant species-specific growth factors, so often human proteins, or indeed bovine serum, are used as a surrogate. It’s advisable to determine the conservation of protein sequence when selecting appropriate growth factors and switch to species-specific proteins if feasible.

  1. Ludwig, T. E. et al. Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185–187 (2006).
  2. Ludwig, T. E. et al. Feeder-independent culture of human embryonic stem cells. Nat. Methods 3, 637–646 (2006).
  3. Chen, G., Gulbranson, D. R., Yu, P., Hou, Z. & Thomson, J. A. Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells. Stem Cells 30, 623–30 (2012).

Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility. To ensure absolute reproducibility and optimize scientific outcomes, all our products rigorously adhere to the Nine-point Qkine Quality Commitment.

This product is only available on orders direct from Qkine. Distributors and resellers of Qkine products are not eligible.

This products is for research use only and not for diagnostic or therapeutic use. 

This product is not for resale.

No additional discount promotion can be applied to the price of the Discovery Kit.

Other restrictions may apply.
Any questions relating to this product should be submitted to customerservice@qkine.com.

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