|recombinant human/mouse/rat Activin A PLUS (Qk005)||£180.00 – £1,900.00|
Human/mouse/rat activin A PLUS protein is an optimised biologically active truncation of the mature domain of human Activin A protein. The EC50 and activity in stem cell culture of activin A PLUS is equivalent to the full mature domain activin A.
High purity 24 kDa dimer comprising truncated mature domain of activin A protein, animal-free (AF) and carrier-protein free (CF). Recombinant activin A PLUS is designed to be manufactured at scale for cost-effective, large-scale stem cell culture applications.
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|recombinant human/mouse/rat follistatin-resistant activin A protein (Qk035)||£120.00 – £180.00|
Follistatin-resistant activin A (FRACTA) has been engineered to prevent binding to the natural inhibitor, follistatin. Activin A activity protein is regulated in vivo by follistatin, a high-affinity inhibitor; follistatin accumulates in stem cell culture, where it inhibits activin A.
Qk035 follistatin-resistant activin A (FRACTA) has equivalent bioactivity to wild-type activin A (Qk001) but does not bind follistatin so is resistant to feedback inhibition. High purity 26 kDa dimer comprising engineered mature domain of activin A protein, animal-free (AF) and carrier-protein free (CF). This specialised activin A has been developed in Marko Hyvönen’s group in the University of Cambridge.
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|recombinant human Gremlin 1 protein (Qk015)||£160.00 – £1,200.00|
Human Gremlin 1 protein is a BMP-inhibitor present in the natural intestinal niche and provides an alternative to Noggin for optimisation of intestinal organoid culture and iPSC differentiation.
Qk015 has been optimized by our experts for exceptionally high-purity production in E.coli and bioactivity. 18 kDa high purity dimeric protein, animal-free and carrier protein free
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|recombinant human HGF (NK1) protein (Qk013)||£160.00 – £1,200.00|
Human HGF (NK1) protein is a potent, high purity NK1 isoform of human hepatocyte growth factor (HGF) that promotes efficient differentiation of human iPSCs to hepatocyte-like cells at just 10 ng/ml with highly homogeneous expression of the hepatic marker, HNF4α. The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.
20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.
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|recombinant human R-spondin 1 protein (Qk006)||£170.00 – £1,600.00|
Human R-spondin 1 protein (RSPO1) is the prototypic member of the R-spondin family and is used to potentiate Wnt signalling in many organoid culture systems including intestinal and tumor (cancer) organoid culture. R-spondin 1 is also required for hematopoietic stem cell specification and cancer cell migration and survival.
13kDa highly pure, bioactive domain of human R-spondin 1 comprising the two cysteine-rich furin-like domains of R-spondin 1, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Qk006 is animal-free and not glycosylated to ensure production of homogeneous protein and enhanced comparability between batches. Used to replace R-spondin conditioned media for improved reproducibility in chemically defined organoid culture media.
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|recombinant human R-spondin 1 LR5 protein (Qk031)||£120.00 – £750.00|
Recombinant R-spondin 1 LR5 protein is engineered to act as a high affinity ligand for the LGR5 receptor. In epithelial tissues LGR5 marks the stem cell population. This engineered protein, R-spondin 1 LR5, activates wnt signalling only in the LGR5+ stem cell population. R-spondin1 LR5 has been tested in intestinal organoid culture and supports organoid survival and growth. As LGR5 specifically marks stem cells and is not found on transit amplifying cells, the lower crypt multiplicity seen in organoid cultures with R-spondin 1 LR5 (in comparison to wild-type R-spondin 1, Qk006) supports the notion that this engineered form is acting specifically on stem cells. This specialized form of R-spondin 1 was developed in Marc de la Roche’s lab (University of Cambridge).
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|recombinant human R-spondin 3 protein (Qk032)||£120.00 – £1,600.00|
Human R-spondin 3 protein potentiates Wnt signalling in and has been shown to function in crypt regeneration in the intestine and control stem cell and progenitor cell behaviour during kidney development. R-spondin 3 is used alongside R-spondin 1 in intestinal organoid culture systems.
17kDa highly pure, bioactive domain of human R-spondin 3 comprising the two cysteine-rich furin-like domains, which are necessary and sufficient for Wnt signalling potentiation and are the essential domains for activity in stem cell and organoid culture. Animal-free and carrier-protein free.
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|recombinant human TGF-β1 PLUS protein (Qk010)||£200.00 – £3,000.00|
Human TGF-β1 PLUS protein is the first entirely animal-free recombinant human transforming growth factor beta 1 (TGF-β1) protein for highly reproducible results and compatible with chemically-defined stem cell media. TGFβ1 is used extensively in E8-style induced pluripotent (iPSC) and embryonic (ESC) stem cell media.
High purity 24 kDa dimer comprising optimised mature domain of TGFβ1 protein, animal-free (AF) and carrier-protein free (CF). Our TGF-β1 PLUS protein has been extensively tested for maintenance of iPSC pluripotency by the specialist stem cell biotechnology company, Stemnovate, Cambridge, UK.
*Benchmark our TGF-β1 PLUS against your existing supplier*
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