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Recombinant mouse noggin protein (Qk033)
Mouse noggin is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids, maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.
Qkine recombinant mouse noggin protein is a highly pure 46 kDa bioactive dimer for reproducible results, animal origin-free (AOF) and carrier-protein free.
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
1000µg will be despatched as 2 x 500µg
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For any questions, please email orders@qkine.com
Summary
High purity mouse noggin protein (Uniprot: P97466)
>98%, by SDS-PAGE quantitative densitometry
46 kDa (dimer)
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in 10 mM HCl at >100 µg/ml (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C
Featured applications
Tumor organoid culture
Epithelial organoid culture (WNR media)
Maintenance of undifferentiated embryonic stem cells
Stem cell differentiation into neural and microglial lineages
Mouse, rat
Bioactivity
Noggin is a BMP inhibitor and its activity was determined by inhibition of BMP-2 (Qk007) activity in a BMP-2 responsive firefly luciferase reporter assay. HEK293T cells were treated (in triplicate) with a serial dilution of noggin and standard concentration of BMP-2 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk033 lot #104284, EC50 = 2.1nM.
Purity
Noggin protein (Qk033) has an unusual migration in non-reduced (NR) SDS-PAGE due to the non-covalent dimer which is the active protein. Similar migration in SDS-PAGE is seen for Gremlin-1, a related BMP antagonist. The identity of the purified dimeric protein was confirmed using mass spectrometry. Upon reduction, the protein monomer migrates at 23 kDa. Purified recombinant mouse noggin protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk033 lot #104284.
Further quality assays
Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine mouse noggin is as biologically active as the comparable alternative supplier protein
Quantitative luciferase assay with Qkine mouse/rat noggin (Qk033, green) and alternative supplier noggin (Supplier B, black) inhibition of BMP-2 (Qk007). Cells were treated in triplicate with a serial dilution of noggin and a standard concentration of BMP-2 for 6 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
Protein background
Noggin is an extracellular antagonist of BMPs (part of the larger TGF-β family); noggin binds and inhibits BMP-2, -4, -5, -7, -13, and -14 with varying affinities [1]. Noggin binds these BMP ligands, preventing interaction of the ligands with their receptor, and consequently blocking downstream activation of signalling cascades.
Through its control of BMP signalling, noggin plays a crucial role in embryonic patterning, as well as in the development of the neural tube, teeth, hair follicles, and the eye [1]. In culture noggin is used in the maintenance of pluripotent stem cells, differentiation of iPSCs to neural lineages, and in a large variety of organoid culture systems including intestine, liver and lung organoids [2].
FAQ
Noggin is a bone morphogenic protein (BMP) inhibitor.
No, noggin is a BMP agonist.
Through binding to BMP and stopping BMP signaling, noggin controls the development of many embryonic structures.
Noggin binds to extracellular BMP, stopping is binding to its receptor.
Noggin can be added to embryonic and induced pluripotent stem cell cultures to preserve pluripotency.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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