Recombinant mouse noggin protein (Qk033)£205.00 – £2,900.00View product
Recombinant mouse noggin protein (Qk033)
£205.00 – £2,900.00
Mouse noggin is a bone morphogenetic protein (BMP) family antagonist used in the culture of intestinal, pancreatic, lung and tumor-derived organoids, maintenance of undifferentiated embryonic stem cells and for stem cell differentiation into neural and microglial lineages.
Qkine recombinant mouse noggin protein is a highly pure 46 kDa bioactive dimer for reproducible results, animal origin-free (AOF) and carrier-protein free.
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Orders are typically shipped same or next day (except Friday).
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£205.00 – £2,900.00
1000µg will be despatched as 2 x 500µg
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Summary:
- High purity mouse noggin protein (Uniprot: P97466)
- 46 kDa (dimer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Tumor organoid culture
Maintenance of undifferentiated embryonic stem cells
Epithelial organoid culture
Stem cell differentiation into neural and microglial lineages
Noggin is a BMP inhibitor and its activity was determined by inhibition of BMP-2 (Qk007) activity in a BMP-2 responsive firefly luciferase reporter assay. HEK293T cells were treated (in triplicate) with a serial dilution of noggin and standard concentration of BMP-2 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk033 lot #104284, EC50 = 62.7 ng/ml (2.1 nM).
Noggin protein (Qk033) has an unusual migration in non-reduced (NR) SDS-PAGE due to the non-covalent dimer which is the active protein. Similar migration in SDS-PAGE is seen for Gremlin-1, a related BMP antagonist. The identity of the purified dimeric protein was confirmed using mass spectrometry. Upon reduction, the protein monomer migrates at 23 kDa. Purified recombinant mouse noggin protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk033 lot #104284.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine mouse noggin is as biologically active as the comparable alternative supplier protein. Quantitative luciferase assay with Qkine mouse noggin (Qk033, green) and alternative supplier noggin (Supplier B, black) inhibition of BMP-2 (Qk007). Cells were treated in triplicate with a serial dilution of noggin and a standard concentration of BMP-2 for 6 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
Technote | Noggin (Qk033) bioactivityProtein background
Noggin is an extracellular antagonist of BMPs (part of the larger TGF-β family); noggin binds and inhibits BMP-2, -4, -5, -7, -13, and -14 with varying affinities [1]. Noggin binds these BMP ligands, preventing interaction of the ligands with their receptor, and consequently blocking downstream activation of signalling cascades.
Through its control of BMP signalling, noggin plays a crucial role in embryonic patterning, as well as in the development of the neural tube, teeth, hair follicles, and the eye [1]. In culture noggin is used in the maintenance of pluripotent stem cells, differentiation of iPSCs to neural lineages, and in a large variety of organoid culture systems including intestine, liver and lung organoids [2].
Additional resources
FAQ
What is noggin?
Noggin is a bone morphogenic protein (BMP) inhibitor.
Is noggin a cytokine or growth factor?
Neither, noggin is a BMP agonist.
What does noggin do?
Through binding to BMP and stopping BMP signaling, noggin controls the development of many embryonic structures.
What does noggin bind to?
Noggin binds to extracellular BMPs, stopping them binding to their receptor.
How is noggin used in cell cultures?
Noggin can be added to embryonic and induced pluripotent stem cell cultures to preserve pluripotency.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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