Recombinant human TGF-β3 protein (Qk054)£255.00 – £3,600.00View product
Recombinant human TGF-β3 protein (Qk054)
£255.00 – £3,600.00
Human transforming growth factor beta 3 (TGF-β3) protein is a member of the TGF-β family, a family involved in regulating cell survival, proliferation and differentiation. TGF-β3 is used in human pluripotent stem cell maintenance media.
Qkine TGF-β3 is a highly pure and bioactive 25.4 kDa protein dimer. Qkine growth factors are animal-origin free (AOF) and carrier-protein free (CF) for reproducible results.
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£255.00 – £3,600.00
1000µg will be despatched as 2 x 500µg
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Summary:
- High purity human TGF-β3 (Uniprot: P10600)
- 12.7 kDa (monomer) 25.4 kDa (dimer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Stem cell expansion and maintenance
Induced pluripotent and embryonic stem cell differentiation and maintenance
iPSC-derived mesoderm differentiation
Frequently used together
Alternative proteins
TGF-β3 activity was determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. EC50 = 50 pg/ml. Bioactivity was determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. Cells were treated (in triplicate) with a serial dilution of TGF-β3 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk054 batch #104369.
TGF-β3 migrates as a single band at 25 kDa in non-reducing (NR) conditions and 13 kDa upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk054 batch #104369.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine TGF-β3 (Qk054) bioactivity was compared with an alternative supplier (Supplier B) using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells (as above). Qkine TGF-β3 and Supplier B were found to have equivalent bioactivity, Qk054 EC50 = 50 pg/ml, Supplier B EC50 = 67 pg/ml (no significant difference).
Technote | TGF beta familyTechnote | TGF-β3 (Qk054) bioactivityProtein background
Transforming growth factor beta 3 (TGF-β3) is a member of the TGF-β family (and TGF-β superfamily). Like other TGF-β family members – TGF-β1 and 2 – TGF-β3 signals via receptor complexes consisting of Type I and Type II serine/threonine kinase receptors, activating intracellular SMAD transcription factors [1]. The TGF-β family have a high degree of overlap/redundancy and regulate a variety of cellular functions, including cell survival, proliferation, and differentiation [2]. TGF-β3 has isoform-specific roles in embryonic palate fusion [3] and wound healing [4].
In culture, TGF-β3 is often used in human pluripotent cell maintenance. TGF-β3 is a component of B8 media – the low-cost weekend-free iPSC media developed in the lab of Paul Burridge, Northwestern University [5]. In the development of B8 media, Kuo et al. proved that while TGF-β1 can maintain iPSCs at 2 ng/ml, TGF-β3 can maintain iPSCs in as little as 0.1 ng/ml, making it a cost-effective alternative to TGF-β1 [5].
Publications using Recombinant human TGF-β3 protein (Qk054)
-
Nutritional requirements of human induced pluripotent stem cells
Lyra-Leite DM, Copley RR, Freeman PP et al.
DOI: doi: 10.1016/j.stemcr.2023.05.004 -
Refined home-brew media for cost-effective, weekend-free hiPSC culture and genetic engineering
Truszkowski L, Bottini S, Bianchi S et al.
DOI: https://doi.org/10.12688/openreseurope.18245.1 -
Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders
Shin D, Kim CN, Ross J et al.
DOI: doi: 10.1016/j.stem.2024.01.010
FAQ
What is TGF beta 3?
TGF-β3 is a growth factor involved in tissue development, wound healing, and immune regulation, crucial for iPSC and ESC proliferation and differentiation.
Where is TGF-β3 found?
TGF-β3 is found in various tissues throughout the body, including the skin, bones, blood vessels, and immune cells.
Is TGF-β3 a cytokine?
Yes, TGF-β3 is a cytokine.
What does the TGF-β3 gene do?
The TGF-β3 gene encodes the transforming growth factor beta 3 protein.
What does TGF-β3 bind to?
TGF-β3 binds to TGF-β receptors. This triggers intracellular signaling pathways that regulate gene expression, influencing cellular processes.
What is the function of the TGF-β3 receptor?
The TGF-β3 receptor serves as a cell surface receptor for the TGF-β3 ligand. It plays a crucial role in transmitting signals from the extracellular environment to the interior of the cell upon ligand binding and initiating intracellular signaling cascades that regulate gene expression.
What is the TGF-β3 pathway?
The TGF-β3 pathway is a series of intracellular signaling events triggered by the binding of TGF-β3 ligands to their cell surface receptors. Upon ligand binding, the TGF-β3 receptors undergo phosphorylation and activation, leading to the activation of downstream signaling molecules such as Smad proteins. These Smad proteins translocate into the nucleus, where they regulate the expression of target genes involved in various cellular processes such as proliferation and differentiation.
How is TGF-β3 used in cell culture?
TGF-β3 regulates cell behavior, promoting differentiation and wound healing. It is often used in tissue engineering, cellular agriculture industries for cultivated meat, and immune modulation.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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