reconstitution solution A (Qk1001)
Solution of 10 mM HCl for use in the reconstitution of lyophilised proteins. For some proteins, physiological buffers may cause precipitation of stock solutions, hence we recommend dissolving our lyophilized TGF beta family and other related growth factors in 10 mM HCl.
reconstitution instructions
- centrifuge briefly before reconstituting
- resuspension in buffer stated on data sheet
- for some proteins physiological buffers may cause precipitation of stock solutions, hence we recommend dissolving our lyophilized TGF beta family and other related growth factors in 10 mM HCl (1:1000 dilution of concentrated HCl), check data sheet for correct reconstitution buffer
- reconstitute to a protein concentration of 0.05–1.0 mg/ml
- allow the reconstituted sample to sit at room temperature for a few minutes before gently agitating
- do not vortex, foaming must be avoided
- for maximum protein recovery of larger sample sizes, reconstitute in approximately 80% of the total volume as above, transfer reconstituted sample to a fresh tube and rinse the original tube with the remaining solution, pool the samples
- our proteins are supplied carrier protein-free, if compatible with your work carrier protein may be added. if you are storing at low concentrations (<50 ug/ml) carrier protein is highly advised
- divide the solution into suitable aliquots, we recommend that single-use aliquots should be prepared to avoid freeze-thaw cycles
reconstitution calculator
desired volume (ug/ml)
volume (ul)
