Recombinant human IGF-1 protein (Qk047) Price range: £60.00 through £190.00View product
Recombinant human IGF-1 protein (Qk047)
Price range: £60.00 through £190.00
Human insulin-like growth factor 1 (IGF-1) protein is used in the maintenance of human pluripotent stem cells (iPSC) and is necessary for cell growth in the absence of insulin. IGF-1 promotes the proliferation of many cell types, including embryonic stem cells (ESC) and mesenchymal stem cells (MSC).
Qkine IGF-1 is a highly pure and bioactive 8 kDa protein, Qkine growth factors are animal origin-free (AOF) and carrier-protein free (CF) for consistent stem cell culture.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £60.00 through £190.00
1000µg will be despatched as 2 x 500µg
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity human IGF-1 protein (Uniprot:P05019)
- 8 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Maintenance of pluripotency in iPSC
Cell culture media optimization and replacement of insulin in defined media
Frequently used together
- Recombinant human activin A protein (Qk001)
- Recombinant human TGF-β1 PLUS™ protein (Qk010)
- Recombinant human NRG-1 protein (Qk045)
- Recombinant porcine EGF protein (Qk064)
- Recombinant human FGF-18 protein (Qk069)
- Recombinant human NT-3 protein (Qk058)
- Recombinant bovine PDGF-BB protein (Qk079)
- Recombinant human FGF-19 protein (Qk086)
- Recombinant human TGF-β2 protein (Qk072)
Related discovery kits
Alternative proteins
IGF-1 activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. EC50 = 3.5 ng/ml (0.45 nM). Cells were treated in triplicate with a serial dilution of IGF-1 for 4 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk047 lot #104331. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340
IGF-1 protein migrates as a single band at 10 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk047 batch #104331.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine IGF-1 remained bioactive in media for 7 days. Bioactivity was determined using an IGF-1-responsive firefly luciferase reporter assay. Transfected HEK293T cells were treated in triplicate for 4 hours with a serial dilution of IGF-1 which had been pre-incubated in conditioned media for 7 days. Firefly luciferase activity was measured and normalised to the control Renilla luciferase activity.
Technote | IGF-1 (Qk047) stabilityProtein background
The insulin-like growth factors (IGFs) are a family of proteins with a similar structure to insulin. IGF-1 has an A and B chain connected by disulphide bonds, its similarity to insulin means it can bind with low affinity to the insulin receptor [1]. IGF-1 is a primary mediator of growth hormone (GH), with growth-promoting effects on most cells and tissues including skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic, and lung [1]. IGF-1 exerts its biological effect through binding to the IGF1 receptor (IGF1R), its effect is modulated through interaction with a group of binding proteins (IGFBPs), which tend to inhibit the effect of IGF-1 [2]. Through IGF1R the Ras-MAPK and PI3 kinase signaling pathways are activated leading to increased cell proliferation and survival [2].
IGF-1 can be used to support maintenance of human embryonic stem cell (ESC) [3] and mesenchymal stem cell (MSC) cultures [3, 4].
Additional resources
- Technote | IGF-1 (Qk047) stability
- Stability of Qkine recombinant growth factors and cytokines in conditioned media (PDF)
- CytoBoostâ„¢ for increased proliferation of muscle and fat cells in serum-free cultures
- CytoBoostâ„¢-enhanced growth factor activity for cell culture and bioprocessing
- Poster: Neural and glial cell maintenance and differentiation
- Poster: Pluripotent stem-cell derived organoids
- Poster: Adult stem-cell derived organoids
- Brochure: Growth factors for neural and glial cell differentiation
- Brochure: Growth factors for enhanced organoid culture protocols
Publications using Recombinant human IGF-1 protein (Qk047)
-
Towards cost-effective and sustainable media formulations for cellular agriculture
Leber R, Rosa J, Laizé V et al.
DOI: doi.org/10.2139/ssrn.5310265
FAQ
What is the IGF-1 receptor pathway?
IGF1R is linked to various cytoplasmic second messenger molecules including the RAS-MAPK and PI3K signaling networks.
What is IGF-1?
IGF-1, also called somatomedin C, is a hormone with a similar molecular structure to insulin.
Where is IGF-1 found?
IGF-1 is primarily produced by the liver.
What does IGF-1 bind to?
IGF-1 binds to the IGF-1 receptor (IGF1R)
How is IGF-1 used in cell culture?
IGF-1 can be used in embryonic stem cell (ESC) and mesenchymal stem cell (MSC) cultures to promote proliferation, as a replacement to insulin.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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