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Recombinant human IGF-1 protein (Qk047)
Human insulin-like growth factor 1 (IGF-1) protein is used in the maintenance of human pluripotent stem cells (IPSC) and is necessary for cell growth in the absence of insulin. IGF-1 promotes the proliferation of many cell types, including embryonic stem cells (ESC) and mesenchymal stem cells (MSC).
Qkine IGF-1 is a highly pure and bioactive 8 kDa protein, Qkine growth factors are animal origin-free (AOF) and carrier-protein free (CF) for consistent stem cell culture.
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1000µg will be despatched as 2 x 500µg
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Summary
High purity human IGF-1 protein (Uniprot:P05019)
>98%, by SDS-PAGE quantitative densitometry
8 kDa
Expressed in E. coli.
Animal origin-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C
Featured applications
Cell culture media optimisation and replacement of insulin in defined media
human
species similarity:
mouse – 94%
rat – 96%
porcine – 100%
bovine – 100%
Alternative
Related discovery kit
Bioactivity
IGF-1 activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected MCF-7 cells. EC50 = 3.5 ng/ml (0.45 nM). Cells are treated in triplicate with a serial dilution of IGF-1 for 4 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk047 lot #104331. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340
Purity
IGF-1 protein migrates as a single band at 10 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 18% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk047 batch #104331.
Further quality assays
Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial >95%
We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine. To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine IGF-1 is stable and bioactive in conditioned media for 7 days
A
B
Stability and bioactivity of Qkine IGF-1 (Qk047). SDS-PAGE of recombinant IGF-1 (A), protein was not degraded when incubated in HEK293T conditioned media for 7 days. Bioactivity was determined using an IGF-1-responsive firefly luciferase reporter assay (B). Transfected HEK293T cells were treated in triplicate for 4 hours with a serial dilution of IGF-1 which had been pre-incubated in conditioned media for 7 days. Firefly luciferase activity was measured and normalised to the control Renilla luciferase activity.
Protein background
The insulin-like growth factors (IGFs) are a family of proteins with a similar structure to insulin. IGF-1 has an A and B chain connected by disulphide bonds, its similarity to insulin means it can bind with low affinity to the insulin receptor [1]. IGF-1 is a primary mediator of growth hormone (GH), with growth-promoting effects on most cells and tissues including skeletal muscle, cartilage, bone, liver, kidney, nerve, skin, hematopoietic, and lung [1]. IGF-1 exerts its biological effect through binding to the IGF1 receptor (IGF1R), its effect is modulated through interaction with a group of binding proteins (IGFBPs), which tend to inhibit the effect of IGF-1 [2]. Through IGF1R the Ras-MAPK and PI3 kinase signaling pathways are activated leading to increased cell proliferation and survival [2].
IGF-1 can be used to support maintenance of human embryonic stem cell (ESC) [3] and mesenchymal stem cell (MSC) cultures [3, 4].
Additional resources
FAQ
IGF-1, also called somatomedin C, is a hormone with a similar molecular structure to insulin.
IGF-1 is primarily produced by the liver.
IGF-1 binds to the IGF-1 receptor (IGF1R)
IGF1R is linked to various cytoplasmic second messenger molecules including the RAS-MAPK and PI3K signaling networks.
IGF-1 can be used in ESC and MSC cultures.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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