Recombinant human gremlin 1 protein (Qk015)£140.00 – £800.00View product
Recombinant human gremlin 1 protein (Qk015)
£140.00 – £800.00
Human gremlin 1 protein is a BMP-inhibitor present in the natural intestinal niche and provides an alternative to noggin for optimisation of intestinal organoid culture and iPSC differentiation.
Qkine gremlin 1 has been optimized by our experts to be an exceptionally high-purity 18 kDa highly bioactive dimeric protein, animal origin-free (AOF) and carrier protein free.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
£140.00 – £800.00
1000µg will be despatched as 2 x 500µg
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Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity human gremlin 1 protein (Uniprot: O60565)
- 18 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Tumor organoid culture
Alternative proteins
Gremlin-1 inhibits BMP-2 induced luciferase activity BRE-HEK293 luciferase reporter assay with an IC50 = 34 ng/ml (1.88 nM). Gremlin 1 activity was determined using inhibition of the BMP-2 response (Qk007 #010, 52 ng/ml) from a BMP2-responsive firefly luciferase reporter in stably transfected HEK293T cells. Cells were treated (n=4) with a serial dilution of gremlin 1 in BMP-2 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk015 batch #011.
Gremlin 1 protein migrates as a single diffuse band at ~36 kDa in non-reducing (NR) and 19 kDa in reducing (R) conditions. The protein is a non-covalent dimer and it is the dissociation of the dimer during electrophoresis which gives the characteristic diffuse band. Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk015 lot #011.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Gremlin 1 protein (GREM1, isoform-1) belongs to the bone morphogenetic protein (BMP) antagonist family. Gremlin 1 protein binds BMP-2, BMP-4, BMP-7 and other BMP family proteins [1] and inhibits receptor binding. It is highly expressed in the small intestine at the base of the intestinal crypts, as are the related proteins, gremlin 2 and chordin-like 1. In this niche, they help maintain the stem cell population by inhibiting BMP produced by mesenchymal cells [2]. Expression of gremlin 1 protein is also detected in fetal brain and colon, and at lower levels in adult brain, prostate, pancreas and skeletal muscle.
Recombinant human gremlin 1 protein and other BMP-antagonists, such as noggin, are used in the derivation, growth and maintenance of organoids from epithelial tissues including intestinal, liver and pancreatic organoids. However, roles for recombinant gremlin 1 protein in cancer stem cell maintenance in glioblastoma have been suggested [3].
Additional resources
Publications using Recombinant human gremlin 1 protein (Qk015)
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Basal delamination during mouse gastrulation primes pluripotent cells for differentiation
Sato N, Rosa VS, Makhlouf A et al.
DOI: doi: 10.1016/j.devcel.2024.03.008
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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