Technote | EGF (Qk011)

Qkine human Epidermal Growth Factor is as biologically active as a comparable alternative supplier protein

Epidermal growth factor (EGF) is involved in the regulation of cell growth and proliferation through the tyrosine kinase receptor EGFR. EGF and the EGFR have been linked to many human diseases, including inflammatory diseases and multiple cancers.

EGF is an essential growth factor for stimulating the proliferation of induced pluripotent stem cells (iPSC) and embryonic stem cells (ESC) and their subsequent differentiation. EGF is also a key component of many media for developing and maintaining organoids.

Qkine EGF (Qk011) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.

Qkine EGF (Qk011) bioactivity

  • Qkine EGF (Qk011) was bioactive in a quantitative luciferase assay with EC50 of 0.11 ng/ml (17.6 pM).
  • This was equivalent to EGF from an alternative supplier, which also had an EC50 of 0.11 ng/ml (17.6 pM).
Qk011_bioassay-comparison

Quantitative luciferase reporter assay shows equivalent bioactivity of Qkine EGF (Qk011, green) and alternative supplier EGF (Supplier B black). HEK293T reporter cells were treated in triplicate with a serial dilution of EGF for 3 hours. Firefly luciferase activity is measured and normalized to control Renilla luciferase activity.

The bioactivity comparison demonstrates that Qkine EGF (Qk011) has equivalent bioactivity to EGF from an alternative major supplier. Qkine EGF (Qk011) has the advantage of being highly pure and animal origin-free, giving batch-to-batch consistency in bioactivity for long-term reproducible culture of stem and organoids.

All our recombinant proteins are animal-free
& come with Bioactivity. Guaranteed.

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Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility.

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Qkine manufactures recombinant proteins in a dedicated animal-free laboratory located in an
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Our dedicated team of stem cell specialists is available to answer any queries and to give expert support when required.