There is no fundamental difference between ESGRO and recombinant mouse leukaemia inhibitor factor (mLIF) protein. Both can be used for the successful maintenance of mouse embryonic stem cell (ESC) pluripotency. 10E7 units of ESGRO is equivalent to 100 µg of pure protein. Thank you to Susanne van den Brink (postdoc in Anna Bigas lab, IMIM, Barcelona) and David Turner (University of Liverpool) for sharing their resuspension protocol to prepare ESLIF media.
June update with featured publication from Austin Smith lab, Kinoshita et al - capture of mouse and human stem cells with features of formative pluripotency; introduction to our "class of 2021" new products we've made this year; explaining pioneering proteins that bring innovative growth factors to stem cell researchers and free reconstitution solution to make lab life easy
We recently held a webinar with CytoSMART and organoid pioneers from Cold Spring Harbor Laboratory, Stemnovate and the Hubrecht Organoid Technology titled: what's needed to realize the potential of organoids? Following presentations from our speakers, we held an insightful Q&A session to address key challenges and questions from the audience. In this blog post, [...]
From standardization of imaging techniques, to more reproducible and cost-effective organoid culture media Organoids have the potential to revolutionize current drug discovery approaches, enhance the physiological relevance of in vitro disease models, and deliver on the promise of precision medicine. But, as with any new technology, there are a number of hurdles to overcome as [...]
Hydrophobic or aromatic? Flexible or rigid? We've designed a handy (and free!) infographic of the amino acid chemical structures and side chain properties. Why not save a copy and hang it up in your lab?