There is no fundamental difference between ESGRO and recombinant mouse leukaemia inhibitor factor (mLIF) protein. Both can be used for the successful maintenance of mouse embryonic stem cell (ESC) pluripotency. 10E7 units of ESGRO is equivalent to 100 µg of pure protein. Thank you to Susanne van den Brink (postdoc in Anna Bigas lab, IMIM, Barcelona) and David Turner (University of Liverpool) for sharing their resuspension protocol to prepare ESLIF media.
We recently held a webinar with CytoSMART and organoid pioneers from Cold Spring Harbor Laboratory, Stemnovate and the Hubrecht Organoid Technology titled: what's needed to realize the potential of organoids? Following presentations from our speakers, we held an insightful Q&A session to address key challenges and questions from the audience. In this blog post, [...]
From standardization of imaging techniques, to more reproducible and cost-effective organoid culture media Organoids have the potential to revolutionize current drug discovery approaches, enhance the physiological relevance of in vitro disease models, and deliver on the promise of precision medicine. But, as with any new technology, there are a number of hurdles to overcome as [...]
Most industries today are under pressure to switch to more ethical and sustainable animal-free alternatives, and now the trend is coming to stem cell labs. As stem cell applications accelerate towards the clinic, novel drug discovery platforms are rapidly scaled, and new transformative stem cell-based technologies such as cultured meat arise, there is [...]
Presentation by Luana Ferrara, Senior Protein Scientist Video script Slide 1: Introduction Hello! My name is Luana Ferrara and I am a senior protein scientist at Qkine. In this short video, I’ll be presenting a comparative analysis of the purity of recombinant Activin A from different suppliers and highlighting why it's important to consider the biochemical quality of growth factors for your stem cell culture. Slide 2: The role of [...]
As a structural biologist, one will very quickly realise that protein not only needs to be pure, but it needs to be also homogeneous and the real proof of the protein’s quality is in its specific bioactivity. So,we clearly need different criteria for defining quality and that is what we do at Qkine.