Can Super Scientist give you your organoid mojo back? Are your growth factors the reason your experiments fail? Can you use engineered or niche-specific growth factors to improve your organoid culture? Super Scientist flies in with easy fixes and questions to ask yourself - do you need to ditch the conditioned media, play around with growth factor combinations, switch noggin and gremlin, or be bold and explore new engineered proteins.
There is no fundamental difference between ESGRO and recombinant mouse leukaemia inhibitor factor (mLIF) protein. Both can be used for the successful maintenance of mouse embryonic stem cell (ESC) pluripotency. 10E7 units of ESGRO is equivalent to 100 µg of pure protein. Thank you to Susanne van den Brink (postdoc in Anna Bigas lab, IMIM, Barcelona) and David Turner (University of Liverpool) for sharing their resuspension protocol to prepare ESLIF media.