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Recombinant bovine PDGF-BB protein (Qk079)
Bovine PDGF-BB (platelet-derived growth factor BB) protein is a homodimer of PDGF-B peptide chains. PDGF-BB can be used for the development of optimized serum-free culture media for species-specific bovine in cellular agriculture protocols. PDGF-BB can be added to bovine culture medium to promote rapid and efficient cell division, maintain high cell proliferation rates in bioreactors, promote the maturation of bovine myoblasts into myotubes and stimulate the production of extracellular matrix components.
Qkine bovine PDGF-BB is a high-purity 24.5 kDa disulfide-linked bioactive dimeric protein that is animal origin-free (AOF) and carrier protein-free. Qkine has optimised the bovine PDGF-BB manufacturing process to produce a highly bioactive species-specific protein with excellent lot-to-lot consistency for enhanced reproducibility.
Orders are typically shipped same or next day (except Friday).
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1000µg will be despatched as 2 x 500µg
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For any questions, please email orders@qkine.com
Summary
High purity bovine protein (Uniprot number: B1H0W5)
>98%, by SDS-PAGE quantitative densitometry
Source: Expressed in E. coli
24.5 kDa monomer
Animal origin-free (AOF) and carrier protein-free
Manufactured in Cambridge, UK
Lyophilized from ACN, TFA
Resuspend in water at >100 µg/mL, prepare single-use aliquots, add carrier protein if desired, and store frozen at -20oC or -80oC
Featured applications
Expansion of bovine mesenchymal stem cells
Cellular agriculture process development
Serum-free media development
Cell expansion
Proliferation of fibroblasts and smooth muscle cells
Tissue repair and regeneration
PDGF-2, Proto-oncogene c-SIS, Becaplermin
bovine
Frequently used together
Recombinant bovine/porcine FGF-2 (154 aa) protein (Qk056)
Recombinant bovine/porcine FGF-2 (145 aa) protein (Qk040)
Recombinant human/bovine/porcine NRG-1 (Qk045)
Recombinant human/bovine/porcine IGF-1 (Qk047)
Recombinant human/bovine/porcine IGF-1 LR3 (Qk041)
Recombinant human/bovine/porcine TGF-β1 PLUS (Qk010)
Recombinant human/mouse/rat/bovine/porcine activin A (Qk001)
Bioactivity
Recombinant bovine PDGF-BB activity was determined using a NFAT reporter assay in HEK293 cells co-transfected with the PDGF β receptor. Transfected cells were treated in triplicate with a serial dilution of bovine PDGF-BB for 6 hours. Firefly activity was measured and normalised to the control Renilla luciferase activity. Data from Qk079 lot 204670.
EC50 = 5.4 ng/mL (221 pM)
Purity
Recombinant bovine PDGF-BB migrates as a band at approximately 14 kDa (monomer) in reduced (R) and as major band at approximately 27 kDa in non-reduced (NR) conditions. No contaminating protein bands are present.
The purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk079 lot 204670.
Further quality assays
Mass spectrometry, single species with the expected mass
Endotoxin: <0.005 EU/μg protein (below the level of detection)
Recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Platelet-derived growth factor-BB (PDGF-BB) is a significant growth factor involved in wound healing and development. It belongs to the PDGF family, which consists of four different polypeptide chains: PDGF-A, PDGF-B, PDGF-C, and PDGF-D [1]. These chains can form homodimers or heterodimers, with PDGF-BB being a homodimer composed of two PDGF-B chains linked by disulfide bonds [2].
PDGF-BB is a protein dimer with a molecular weight of 24.5 kDa. The structure includes a conserved cystine knot motif, which is crucial for its stability and function [1,2,3]. The dimerization of PDGF-BB is mediated by
specific disulfide bonds between cysteine residues. This dimeric structure is essential for its biological activity, enabling the molecule to effectively bind to its receptors [3].
PDGF-BB primarily functions by binding to PDGF receptors (PDGFR-α and PDGFR-β), which are tyrosine kinase receptors located on the cell surface. Upon binding, PDGF-BB induces receptor dimerization and autophosphorylation, initiating a cascade of downstream signaling pathways. These pathways include the PI3K/Akt, MAPK, and PLCγ pathways, which regulate various cellular responses such as proliferation, migration, survival, and differentiation [4].
PDGF-BB stimulates the proliferation and migration of fibroblasts and smooth muscle cells, promoting the formation of granulation tissue and the remodeling of extracellular matrix components [5]. PDGF-BB enhances angiogenesis by recruiting pericytes and smooth muscle cells to stabilize newly formed blood vessels [4.5].
In cultivated meat manufacturing, Platelet-Derived Growth Factor-BB (PDGF-BB) is essential for enhancing cell proliferation, differentiation, and tissue formation. During initial cell culture and expansion in bioreactors, PDGF-BB promotes rapid cell division, ensuring a sufficient cell mass. It aids in the differentiation of myoblasts into mature muscle cells and improves cell migration and integration within tissue scaffolds, resulting in more structured and functional meat tissue. PDGF-BB also stimulates extracellular matrix production, enhancing the structural and mechanical properties of the cultivated meat. By providing specific growth signals, PDGF-BB can reduce the reliance on fetal bovine serum (FBS), making the process more cost-effective and ethically acceptable. The role of PDGF-BB in cell growth and tissue engineering leads to more efficient production and higher-quality cultivated meat that closely resembles conventional meat in texture and nutritional content [6,7].
FAQ
PDGF-BB (Platelet-Derived Growth Factor-BB) is a homodimeric protein growth factor that plays a key role in wound healing and tissue regeneration by activating cellular responses through binding to PDGF receptors.
PDGF-BB is found in platelets and other various cell types, where it plays a crucial role in regulating cellular functions and tissue repair processes.
Yes, PDGF-BB is considered a cytokine. Cytokines are small proteins that are important in cell signaling, especially in the immune system. PDGF-BB fits into this category as it regulates cell growth and division, particularly in the context of wound healing and tissue repair.
The PDGF-BB gene encodes a protein crucial for wound healing and tissue repair. PDGF-BB acts by binding to PDGF receptors on cell surfaces, initiating signaling pathways that promote cell growth, migration, and survival. This process facilitates the formation of granulation tissue and supports angiogenesis, essential for restoring tissue integrity.
The PDGF-BB receptor, a receptor tyrosine kinase (PDGFR-α and PDGFR-β), mediates cellular responses to PDGF-BB.
The PDGF-BB pathway refers to the sequence of events initiated when PDGF-BB binds to its receptors (PDGFR-α and PDGFR-β). This binding activates receptor tyrosine kinases, triggering downstream signaling cascades such as PI3K/Akt, MAPK, and PLCγ pathways.
PDGF-BB is commonly used in cell culture to stimulate cell proliferation, particularly of mesenchymal cells such as fibroblasts and smooth muscle cells. It is added to the culture medium at specific concentrations to mimic its physiological effects, promoting cell growth and maintaining cellular phenotype in vitro.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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