Recombinant bovine BMP-4 protein (Qk135) Price range: £250.00 through £3,190.00View product
Recombinant bovine BMP-4 protein (Qk135)
Price range: £250.00 through £3,190.00
BMP-4 (bone morphogenetic protein 4) is a key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells. In addition, BMP-4 protein plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, and regulating neuronal and glial cell development.
Qkine BMP-4 is a highly pure, animal origin-free and carrier protein-free 24 kDa disulfide–linked bioactive dimer comprised of the mature domain of bovine BMP-4 protein.
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Price range: £250.00 through £3,190.00
500 ug shipped as 5 x 100 ug | 1000 ug shipped as 10 x 100 ug
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Summary:
- High purity optimized human BMP-4 protein (Uniprot: Q2KJH1)
- 24 kDa (dimer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Related discovery kits
BMP-4 activity was determined using the BMP4-responsive firefly luciferase reporter assay in transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of BMP-4 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 30.2 pM (0.72 ng/ml).
BMP-4 dimer migrates as a single band at 24 kDa in non-reducing (NR) and 13 kDa as a single monomeric species upon reduction (R). High purity yield of dimeric protein (bioactive form). Purified recombinant protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.05 EU/μg protein
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine BMP-4 has higher bioactivity than bacterial and mammalian-expressed BMP-4 from alternative suppliers. Quantitative luciferase assay with Qkine BMP-4 (green) and BMP-4 from alternative suppliers (Supplier A, blue; Supplier B, black). Cells were treated in triplicate with a serial dilution of BMP-4 for 6 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
Product background
Bone morphogenetic protein 4 (BMP-4) is part of the TGF-β superfamily of growth factors. BMP-4 is a key regulator of embryogenesis with roles in gastrulation, establishment of a dorsal-ventral axis, and regulation of limb development [1]. As their name suggest the BMP family is involved in the development and maintenance of bone and cartilage; in adults BMP-4 has roles bone and cartilage metabolism, as well as neurogenesis [2] and hematopoietic stem cell homing [3].
BMP-4 protein acts through two serine-threonine kinase receptors; Bone morphogenetic protein receptor (BMPR) 1 and BMPR2, via these receptors BMP-4 invokes Smad and map kinase pathways and consequently alters target gene transcription [1].
Recombinant BMP-4 is an essential growth factor for differentiation of induced pluripotent (iPSC) and embryonic stem cells to mesoderm lineage for further differentiation to cardiac [4, 5] and stromal cells [5]. BMP-4 is also used in a wide range of stem cell and organoid differentiation protocols. It is used in choroidal and melanocyte differentiation [6] and intestinal organoids [7]. BMP-4 has a morphogenetic effects in spinal organoids and modulates the arrangement and concentration of dorsal neural tube cell types in these organoids [8].
Additional resources
FAQ
What is BMP-4?
BMP-4 is a TGF-beta family protein essential for development, particularly eye, heart and cranial formation. It is also essential in many stem cell and organoid differentiation media related to mesodermal lineage.
Where is BMP-4 found?
BMP-4 is expressed by a wide range of cells and tissues through the body and expression and effect on different tissues varies dependent on the developmental stage. BMP-4 is particularly expressed by endothelial cells, mesenchymal cells, epithelial cells and adipocytes.
Is BMP-4 a cytokine?
Yes, BMP-4 is a TGF-beta family cytokine.
What is the function of the BMP-4 receptor?
The activated type I receptor phosphorylates and activates receptor-regulated Smads (R-SMADs), which are SMAD1, SMAD5, and SMAD8 proteins. BMP-4 can also activate the p38 mitogen-activated protein kinase (MAPK) pathway, in addition to the Smad pathway.
What is the BMP-4 pathway?
BMP-4 activates the Smad and MAPK pathways to effect transcription of target genes.
What does BMP-4 bind to?
BMPs induce specific cellular responses through their interaction with type I and type II cell surface receptors, which activates the serine/threonine kinase activity of the receptors 5. Three distinct BMP type II receptors (BMPRIIs) and four BMP type I receptors (BMPRIs), also termed activin receptor‐like kinases (ALKs), have been characterized. BMP-2 and BMP-4 bind most strongly to ALK3 and ALK6.
What are BMP-4 antagonists?
BMP pathways are actively regulated by BMP antagonists including chordin, noggin and gremlin, these bind to BMP-4 and prevent it from binding to its receptors.
How is BMP-4 used in cell culture?
BMP-4 is widely used in stem cell and organoid differentiation protocols as well as the study of body patterning and development.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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