Dopaminergic neuron differentiation kit (Qk517) Original price was: £865.00.£505.00Current price is: £505.00.
Dopaminergic neuron differentiation kit (Qk517)
Original price was: £865.00.£505.00Current price is: £505.00.
For validating the ability of induced pluripotent stem (iPSC) lines to differentiate into dopaminergic neurons.
The dopaminergic neuron differentiation kit is a comprehensive tool designed to assess and confirm the differentiation potential of both newly derived and established iPSC lines. This kit enables researchers to evaluate the ability of iPSCs to differentiate into dopaminergic neurons, a specialized neuronal subtype essential for motor control, reward processing, and cognitive functions. Dopaminergic neurons play a crucial role in neurological health, and their degeneration is a hallmark of Parkinson’s disease.
The kit includes carefully optimized growth factors required to efficiently guide iPSCs toward the dopaminergic neurons. Each kit is sufficient for differentiation of 2x 96 well plates for 35 days.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Original price was: £865.00.£505.00Current price is: £505.00.
1000µg will be despatched as 2 x 500µg
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
>98%, by SDS-PAGE quantitative densitometry
Animal origin-free (AOF) and carrier protein-free
Expressed in E. coli
Bioactivity Guaranteed
Manufactured in our Cambridge, UK laboratories
Lyophilized
- See reconstitution instructions for individual kit components
Featured applications:
Differentiation of midbrain dopaminergic neurons
Human BDNF – Qk050 – 25 µg
Used to maintain neurons and differentiate and mature human pluripotent stem cell-derived neural progenitors to cortical and motor neurons and cortical organoids.
Human GDNF – Qk051 – 25 µg
Used to maintain neurons and cortical organoids and to differentiate dopaminergic neurons from human pluripotent stem cell-derived neural progenitors. GDNF also facilitates the differentiation of neural progenitors to astrocytes.
Human TGF-ß3 – Qk054 – 25 µg
Member of the TGF-β family, a family involved in regulating cell survival, proliferation and differentiation. TGF-β3 is used in human pluripotent stem cell maintenance media.
Human FGF-8a – Qk059 – 25 µg
Often used for the differentiation of induced pluripotent stem cells, embryonic stem cells, and neural stem cells.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Dopaminergic neurons are specialized nerve cells responsible for the synthesis, storage, and release of the neurotransmitter dopamine. These neurons play a pivotal role in several critical brain functions, including motor control, reward, motivation, and regulation of mood [1]. Dopaminergic neurons are primarily located in the substantia nigra and the ventral tegmental area of the brain. They project to various brain regions, including the striatum, prefrontal cortex, and limbic system, forming complex networks that influence a variety of behaviors and physiological processes [2].
Dopaminergic neurons are crucial in motor control, as evidenced by their degeneration in Parkinson’s disease, a condition characterized by motor symptoms such as tremors, rigidity, and bradykinesia. In psychiatric disorders, dysregulation of dopaminergic signaling is implicated. For instance, hyperactivity of dopaminergic pathways is associated with the positive symptoms of schizophrenia, while diminished dopamine function is linked to anhedonia and lack of motivation seen in depression [2]. Differentiating iPSCs into dopaminergic neurons is an area of research with significant implications for treating neurological disorders, especially Parkinson’s disease.
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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