• Granulocyte colony-stimulating factor (G-CSF) is a member of the hematopoietic growth factor family which plays a crucial role in the proliferation, differentiation, and maturation of committed progenitor cells to granulocytes, such as neutrophils. G-CSF is produced by various cell types including macrophages, fibroblasts, and endothelial cells. It binds to the G-CSF surface receptors upon stimulation to activate key cellular processes involved in neutrophil proliferation, maturation, and mobilization in healthy and diseased states.G-CSF is commonly used in vitro to maintain neutrophils and stimulate the differentiation of cells to neutrophil lineage. is a highly pure, bioactive glycoprotein produced in an animal-free expression system composed of 174 amino acids with a molecular weight of 18.6 kDa. Human and murine G-CSF are 73% identical at the amino acid level and show species cross-reactivity. Human G-CSF is carrier-free, tag-free, and non-glycosylated to ensure a pure and homogenous production of high-quality neutrophils and other relevant cell cultures with exceptional lot-to-lot consistency.
  • Recombinant bovine HGF protein is a potent, high-purity NK1 isoform of bovine hepatocyte growth factor (HGF).   Bovine HGF differs from human HGF at several key amino acid residues that are predicted to affect bioactivity and receptor binding.  Use of species-specific HGF growth factor will facilitate media optimization for cellular agriculture (cultivated meat) and veterinary applications. Also available is Qk061 Porcine HGF (NK1). The highly scalable animal-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.20kDa naturally occurring isoform of HGF, animal-free and carrier-protein free.
  • Sonic hedgehog protein (Shh) is a member of the Hedgehog family with an essential role in embryonic development, tissue regeneration, and tumorigenesis. Shh induces the cell fate and patterning of neural progenitors in ventral domains at various levels in the forebrain, midbrain, hindbrain, and spinal cord. It has many applications in the neural stem cell field where it plays a significant role in differentiating human-induced pluripotent stem cells (iPSC) towards motor neurons and interneurons. Also, it induces the patterning of organoids and embryos in culture.Recombinant Shh protein has a molecular weight of 19.8kDa. This protein is animal-free (AOF), carrier protein-free, and tag-free to ensure its purity with exceptional lot-to-lot consistency. Sonic hedgehog protein is suitable for the culture of reproducible and high-quality neurons and organoids.
  • Recombinant human r-spondin 1 LR5 protein is engineered to act as a high affinity ligand for the LGR5 receptor.  In epithelial tissues LGR5 marks the stem cell population.  This engineered protein, R-spondin 1 LR5, activates wnt signalling only in the LGR5+ stem cell population.  R-spondin1 LR5 has been tested in intestinal organoid culture and supports organoid survival and growth. As LGR5 specifically marks stem cells and is not found on transit amplifying cells, the lower crypt multiplicity seen in organoid cultures with R-spondin 1 LR5 (in comparison to wild-type R-spondin 1, Qk006) supports the notion that this engineered form is acting specifically on stem cells.  This specialized form of R-spondin 1 was developed in Marc de la Roche’s lab (University of Cambridge).

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