Currency

USD: $ ^
Sale
Dopaminergic neuron differentiation kit (Qk517)

Dopaminergic neuron differentiation kit (Qk517)

For validating the ability of induced pluripotent stem (iPSC) lines to differentiate into dopaminergic neurons.

The dopaminergic neuron differentiation kit is a comprehensive tool designed to assess and confirm the differentiation potential of both newly derived and established iPSC lines. This kit enables researchers to evaluate the ability of iPSCs to differentiate into dopaminergic neurons, a specialized neuronal subtype essential for motor control, reward processing, and cognitive functions. Dopaminergic neurons play a crucial role in neurological health, and their degeneration is a hallmark of Parkinson’s disease.

The kit includes carefully optimized growth factors required to efficiently guide iPSCs toward the dopaminergic neurons. Each kit is sufficient for differentiation of 2x 96 well plates for 35 days.

Fast and free worldwide shipping.
Orders are typically shipped same or next day (except Friday).

Buy online with secure credit card or purchase order. You can also place your order by email orders@qkine.com

Kit contents

  • Human BDNF – Qk050 – 25 µg
    Used to maintain neurons and differentiate and mature human pluripotent stem cell-derived neural progenitors to cortical and motor neurons and cortical organoids.

  • Human GDNF – Qk051 – 25 µg
    Used to maintain neurons and cortical organoids and to differentiate dopaminergic neurons from human pluripotent stem cell-derived neural progenitors. GDNF also facilitates the differentiation of neural progenitors to astrocytes.

  • Human TGF-ß3 – Qk054 – 25 µg
    Member of the TGF-β family, a family involved in regulating cell survival, proliferation and differentiation. TGF-β3 is used in human pluripotent stem cell maintenance media.

  • Human FGF-8a – Qk059 – 25 µg
    Often used for the differentiation of induced pluripotent stem cells, embryonic stem cells, and neural stem cells.

Quality features

  • Highly pure >98%, by SDS-PAGE quantitative densitometry

  • Highly bioactive – come with our Bioactivity Guarantee

  • Source: Expressed in E. coli 

  • Animal origin-free (AOF) and carrier protein-free

  • Manufactured in Cambridge, UK

  • Lyophilized

Quality assays

  • Mass spectrometry, single species with the expected mass

  • Endotoxin: <0.005 EU/μg protein (below the level of detection)

  • Recovery from stock vial: >95%

Background

Dopaminergic neurons are specialized nerve cells responsible for the synthesis, storage, and release of the neurotransmitter dopamine. These neurons play a pivotal role in several critical brain functions, including motor control, reward, motivation, and regulation of mood [1]. Dopaminergic neurons are primarily located in the substantia nigra and the ventral tegmental area of the brain. They project to various brain regions, including the striatum, prefrontal cortex, and limbic system, forming complex networks that influence a variety of behaviors and physiological processes [2].

Dopaminergic neurons are crucial in motor control, as evidenced by their degeneration in Parkinson’s disease, a condition characterized by motor symptoms such as tremors, rigidity, and bradykinesia. In psychiatric disorders, dysregulation of dopaminergic signaling is implicated. For instance, hyperactivity of dopaminergic pathways is associated with the positive symptoms of schizophrenia, while diminished dopamine function is linked to anhedonia and lack of motivation seen in depression [2]. Differentiating iPSCs into dopaminergic neurons is an area of research with significant implications for treating neurological disorders, especially Parkinson’s disease.

[1] O Klein, M. et al. Dopamine: Functions, Signaling, and Association with Neurological Diseases. Cellular and Molecular Neurobiology. 2019 Jan;39(1):31-59. doi: 10.1007/s10571-018-0632-3

[2] Zhuang, Y. et al. Mechanism of dopamine binding and allosteric modulation of the human D1 dopamine receptor. Cell Research 2021 May;31(5):593-596. doi.org/10.1038/s41422-021-00482-0

Additional information

Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility. To ensure absolute reproducibility and optimize scientific outcomes, all our products rigorously adhere to the Nine-point Qkine Quality Commitment.

Qkine products are intended solely for research purposes and ex vivo cell manufacturing. Not for therapeutic or diagnostic use.
Discovery kits are only available on orders direct from Qkine. Distributors and resellers of Qkine products are not eligible. This product is not for resale.
No additional discount promotion can be applied to the price of the Discovery Kit. Other restrictions may apply.
If you have any questions relating to this product, please email customerservice@qkine.com.

Share your thoughts!

Let us know what you think...

Leave a Review Ask a Question Post a Photo

What others are saying

There are no contributions yet.

×

Login

Register


Your personal data will be used to support your experience throughout this website, to manage access to your account, and for other purposes described in our privacy policy.

Continue as a Guest

Don't have an account? Sign Up

Continue as a Guest

Amazon gift voucher available for growth factor and cytokine reviews

Receive an Amazon gift voucher when you leave us a review.

£25, $30 or €30 for reviews with an image and £10, $15 or €15 for reviews without an image
Terms and conditions apply