Technote | HGF, OSM, BMP-4, FGF-2, and Activin A (Qk001Qk038Qk053Qk013Qk049)
Hepatocellular differentiation with Qkine animl-free growth factors
Hepatocyte-like cells (HLCs) derived from iPSCs exhibit many functions of primary liver cells. This advancement holds promise for liver disease research and the development of personalized treatment pathways. These advancements may ease the demand for liver transplants by providing an alternative cell source for therapeutic use. Furthermore, HLCs are powerful models for studying liver metabolism, genetic liver diseases, and drug interactions, which are vital for evaluating drug safety and liver-specific toxicities before clinical trials.
Differentiation of iPSC to hepatocyte-like cells using Qkine growth factors
- Producing functional hepatocytes from iPSCs requires precise activation of key signaling pathways, such as Wnt, activin/nodal, FGF, and BMP, to replicate the stages of liver development. The sequential activation of these pathways supports early liver cell differentiation, while maturation is driven by factors such as hepatocyte growth factor (HGF) and oncostatin M (OSM).
- Qkine growth factors provide the only completely animal-free complete workflow for hepatocyte differentiation.
Immunocytochemistry of hepatocellular markers in differentiated iPSCs. Albumin [green, A], Hoechst 33258 [blue, B], combined albumin and Hoechst [C], Alpha-1-fetoprotein [green, D], Hoechst 33258 [blue, E], combined alpha-1-fetoprotein and Hoechst [F], and alpha 1 antitrypsin [green, G], Hoechst 33258 [blue, H], alpha 1 antitrypsin and Hoechst [I]. Images were acquired using the EVOS M5000 system (scale bar = 150 µm).
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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