Recombinant mouse TGF-β3 protein (Qk019) Price range: £320.00 through £3,960.00View product
Recombinant mouse TGF-β3 protein (Qk019)
Price range: £320.00 through £3,960.00
Transforming growth factor beta 3 (TGF-β3) protein is a member of the TGF-β family, a family involved in regulating cell survival, proliferation and differentiation. TGF-β3 is used in pluripotent stem cell maintenance media.
Qkine mouse TGF-β3 is a highly pure and bioactive 25.4 kDa protein dimer. Qkine growth factors are animal-origin free (AOF) and carrier-protein free (CF) for reproducible results.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £320.00 through £3,960.00
1000 µg will be dispatched as 2 x 500 µg.
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity TGF-β3 (Uniprot: P17125)
- 12.7 kDa (monomer) 25.4 kDa (dimer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Expansion and differentiation of mouse pluripotent stem cells
Propagation of mouse embryonic stem cells
iPSC-derived mesoderm differentiation
TGF-β3 activity was determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. EC50 = 50 pg/ml. Bioactivity was determined using a TGF-β3 responsive firefly luciferase reporter in HEK293T cells. Cells were treated (in triplicate) with a serial dilution of TGF-β3 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk054 batch #104369.
TGF-β3 migrates as a single band at 25 kDa in non-reducing (NR) conditions and 13 kDa upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk054 batch #104369.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.05 EU/μg protein
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Transforming growth factor beta 3 (TGF-β3) is a member of the TGF-β family (and TGF-β superfamily). Like other TGF-β family members – TGF-β1 and 2 – TGF-β3 signals via receptor complexes consisting of Type I and Type II serine/threonine kinase receptors, activating intracellular SMAD transcription factors [1]. The TGF-β family have a high degree of overlap/redundancy and regulate a variety of cellular functions, including cell survival, proliferation, and differentiation [2]. TGF-β3 has isoform-specific roles in embryonic palate fusion [3] and wound healing [4].
In culture, TGF-β3 is often used in pluripotent cell maintenance. TGF-β3 is a component of B8 media – the low-cost weekend-free iPSC media developed in the lab of Paul Burridge, Northwestern University [5]. In the development of B8 media, Kuo et al. proved that while TGF-β1 can maintain iPSCs at 2 ng/ml, TGF-β3 can maintain iPSCs in as little as 0.1 ng/ml, making it a cost-effective alternative to TGF-β1 [5].
Mouse TGF-β3 has 100% homology to human TGF-β3 (Qk054).
FAQ
Special guidance for reconstitution of TGF beta family including activins, BMPs and GDF-15
TGF beta family proteins and other growth factors can be very poorly soluble in physiological solutions. Please follow the handling guidance for lyophilized cytokines below to minimize loss of protein due to precipitation or adsorption to plastic. We advise storing the recombinant protein at very low pH before dilution in cell culture media or final working solutions. Low pH will also assist in maintaining the correct disulfide structure of the protein by minimizing disulfide bond exchange reactions.
- Resuspension in physiological buffers may cause precipitation of stock solutions, hence we recommend dissolving our lyophilized cytokines in 10 mM HCl (1:1000 dilution of concentrated HCl) while keeping the protein concentration at 50 µg/ml or above, in order to avoid loss by adsorption to plasticware.
- To ensure you recover all of the protein, let the sample sit for a few minutes with the solubilization buffer at room temperature and pipette gently up and down (avoid foaming).
- Rinse the tube with some more 10 mM HCl and pool with the rest.
- The protein is tolerant of some freeze and thaw cycles, but as always with proteins, it is better to aliquot and store frozen.
- Our proteins are supplied carrier-protein free. If compatible with your work, add carrier protein of your choice such as BSA, HSA or gelatin to further minimize loss by adsorption.
- Store in -80°C for long term storage. -20°C for short-term.
Every effort is made to ensure samples are sterile; however, we recommend sterile filtering after dilution in media or the final working solution.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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