Leukemia Inhibitory Factor (LIF) belongs to the IL-6 cytokine family and regulates embryonic development, immune response, and inflammation. LIF is essential to maintain stem cell pluripotency, so it is widely used in embryonic and induced pluripotent stem cell cultures. Leukemia inhibitory factor can also differentiate and maintain immune cells, such as T cells and macrophages.
Animal-free growth factors can ensure that cell cultures are reproducible and physiologically relevant as they have higher lot-to-lot consistency and eliminate contamination from animal-derived ingredients. Qkine manufactures an animal-free, carrier-free, and tag-free LIF to ensure high and consistent bioactivity. This technote demonstrates a comparable level of bioactivity between Qkine leukemia inhibitory factor and an alternative major supplier of bacterial-expressed LIF (Supplier B).