Recombinant human KGF (FGF-7) protein (Qk046) Price range: £290.00 through £3,600.00View product
Recombinant human KGF (FGF-7) protein (Qk046)
Price range: £290.00 through £3,600.00
Human KGF protein (FGF-7) is a member of the FGF family. Recombinant KGF protein is used in the culture of lung, breast, liver, and bladder organoids.
Qkine human KGF (FGF-7) is a highly pure and bioactive 19 kDa protein, animal-origin free (AOF) and carrier-protein free (CF).
Qkine KGF (FGF-7) is also available as cell therapy grade with extended quality testing and documentation – Qk046-CTG
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £290.00 through £3,600.00
1000µg will be despatched as 2 x 500µg
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity KGF (FGF-7) protein (Uniprot: P21781)
- 19 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from HEPES, NaCl, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Airway, mammary, liver hepatocyte, and urothelium organoids
Alternative proteins
KGF (FGF-7) activity was determined using the Promega serum response element luciferase reporter assay in transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of KGF for 4 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 1.75 ng/ml (92 pM). Data from Qk046 lot #104314.
KGF migrates as a monomer at 19 kDa and a dimer at 38 kDa in non-reducing (NR) conditions and 19 kDa upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk046 lot #104314.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
KGF (FGF-7) from Qkine (Qk046) and a major supplier have equivalent bioactivity. Qk046 KGF (FGF-7) lot #104425 and KGF from another supplier were compared in the same assay plate with EC50s of 2.0 ng/ml and 3.7 ng/ml respectively.
Technote | KGF (FGF-7) (Qk046) bioactivityProtein background
Keratinocyte growth factor (KGF), also known as fibroblast growth factor-7 (FGF-7), is part of the large FGF family, and, alongside FGF-3, FGF-10, and FGF-22, is a member of the FGF-7 subfamily. This subfamily is secreted exclusively by the mesenchyme, and specifically activates the “b” isoforms of FGFR1 (FGFR1b) and FGFR2 (FGFR2b) present in the overlying epithelium [1], of these receptors KGF acts through the IIIb splice form of FGFR2. KGF is required for the development of the kidney, thymus, and hippocampus [2]. KGF also plays an import role in wound healing [3], a role exemplified in studies on bioengineered skin [3]. KGF protein has been used in generation of many organoids including lung, breast, liver and bladder [2].
Publications using Recombinant human KGF (FGF-7) protein (Qk046)
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Generation of human iPSC-derived pancreatic organoids to study pancreas development and disease
Darrigrand J-F, Isaacson A and Spagnoli FM
DOI: DOI:10.12688/f1000research.162496.1
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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