Recombinant human KGF (FGF-7) protein (Qk046)£255.00 – £3,600.00View product
Recombinant human KGF (FGF-7) protein (Qk046)
£255.00 – £3,600.00
Human KGF protein (FGF-7) is a member of the FGF family. Recombinant KGF protein is used in the culture of lung, breast, liver, and bladder organoids.
Qkine human KGF (FGF-7) is a highly pure and bioactive 19 kDa protein, animal-origin free (AOF) and carrier-protein free (CF).
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Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
£255.00 – £3,600.00
1000µg will be despatched as 2 x 500µg
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Summary:
- High purity KGF (FGF-7) protein (Uniprot: P21781)
- 19 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from PBS
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Airway, mammary, liver hepatocyte, and urothelium organoids
KGF (FGF-7) activity was determined using the Promega serum response element luciferase reporter assay in transfected MCF-7 cells. Cells were treated in triplicate with a serial dilution of KGF for 4 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 1.75 ng/ml (92 pM). Data from Qk046 lot #104314.
KGF migrates as a monomer at 19 kDa and a dimer at 38 kDa in non-reducing (NR) conditions and 19 kDa upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk046 lot #104314.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
KGF (FGF-7) from Qkine (Qk046) and a major supplier have equivalent bioactivity. Qk046 KGF (FGF-7) lot #104425 and KGF from another supplier were compared in the same assay plate with EC50s of 2.0 ng/ml and 3.7 ng/ml respectively.
Technote | KGF (FGF-7) (Qk046) bioactivityProtein background
Keratinocyte growth factor (KGF), also known as fibroblast growth factor-7 (FGF-7), is part of the large FGF family, and, alongside FGF-3, FGF-10, and FGF-22, is a member of the FGF-7 subfamily. This subfamily is secreted exclusively by the mesenchyme, and specifically activates the “b” isoforms of FGFR1 (FGFR1b) and FGFR2 (FGFR2b) present in the overlying epithelium [1], of these receptors KGF acts through the IIIb splice form of FGFR2. KGF is required for the development of the kidney, thymus, and hippocampus [2]. KGF also plays an import role in wound healing [3], a role exemplified in studies on bioengineered skin [3]. KGF protein has been used in generation of many organoids including lung, breast, liver and bladder [2].
Additional resources
Publications using Recombinant human KGF (FGF-7) protein (Qk046)
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Generation of human iPSC-derived pancreatic organoids to study pancreas development and disease
Darrigrand J-F, Isaacson A and Spagnoli FM
DOI: https://doi.org/10.1101/2024.10.24.620102
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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