Hepatocellular-like differentiation kit (Qk518) Original price was: £5,760.00.£2,850.00Current price is: £2,850.00.
Hepatocellular-like differentiation kit (Qk518)
Original price was: £5,760.00.£2,850.00Current price is: £2,850.00.
For differentiation of induced pluripotent stem cells (iPSCs) into hepatocyte-like cells (HLCs).
The hepatocellular-like differentiation kit is a comprehensive tool designed to assess and confirm the differentiation potential of both newly derived and established iPSC lines. HLCs derived from iPSCs exhibit many functions of primary liver cells for liver disease research and the development of personalized treatment pathways. HLCs are powerful models for studying liver metabolism, genetic liver diseases, and drug interactions, which are vital for evaluating drug safety and liver-specific toxicities before clinical trials.
The kit includes carefully optimized growth factors required to efficiently guide iPSCs into HLCs. Each kit is sufficient for differentiation of 1 x 6 well plates for 21 days.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Original price was: £5,760.00.£2,850.00Current price is: £2,850.00.
1000µg will be despatched as 2 x 500µg
Fast and free shipping.
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
>98%, by SDS-PAGE quantitative densitometry
Animal origin-free (AOF) and carrier protein-free
Expressed in E. coli
Bioactivity Guaranteed
Manufactured in our Cambridge, UK laboratories
Lyophilized
- See reconstitution instructions for individual kit components
Featured applications:
Differentiation of iPSCs to hepatocyte-like cells
Human activin A – Qk001 – 25 µg
Frequently used to maintain pluripotency in induced pluripotent and embryonic stem cell cultures. It is also used in many stem cell differentiation protocols, including endoderm lineage differentiation and further maturation into hepatocyte and pancreatic cells.Â
Human HGF NK1 – Qk013 – 1×500 µg, 1×1000 µg
HGF NK1 protein is a potent naturally occurring isoform of HGF it promotes efficient differentiation of human induced pluripotent stem cells to hepatocyte-like cells with highly homogeneous expression of the hepatic marker.
Human BMP-4 – Qk038 – 25 µg
A key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells.
Human OSM – Qk049 – 1000 µg
Qkine human oncostatin M (OSM) protein is high purity and animal origin-free (AOF), used in the differentiation of human pluripotent stem cells into hepatocyte-like cells.
Human FGF2-G3 (154 aa) – Qk053 – 50 µg
A thermostable engineered form of human FGF-2. Human FGF2-G3 154 aa is the 154 aa mature domain of FGF-2. The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3).
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Since their introduction in 2006, induced pluripotent stem cells (iPSCs) have revolutionized research and therapeutic development. iPSCs are invaluable for disease modeling due to their unique ability to differentiate into nearly any cell type, making them a powerful tool for studying genetic disorders, infections, and cancers. Their role in high-throughput drug screening and toxicity studies has significantly advanced our understanding of disease mechanisms across various conditions and enabling the customization of treatment strategies based on individual genetic profiles [1]. Additionally, iPSCs offer an ethical and sustainable alternative to embryonic stem cells, further broadening their application in research and regenerative medicine.
One key application of iPSCs is hepatocellular differentiation, a critical area within regenerative medicine. Hepatocyte-like cells (HLCs) derived from iPSCs exhibit many functions of primary liver cells. This advancement holds promise for liver disease research and the development of personalized treatment pathways. These advancements may ease the demand for liver transplants by providing an alternative cell source for therapeutic use [2]. Furthermore, HLCs are powerful models for studying liver metabolism, genetic liver diseases, and drug interactions, which are vital for evaluating drug safety and liver-specific toxicities before clinical trials.
Producing functional hepatocytes from iPSCs requires precise activation of key signaling pathways, such as Wnt, activin/nodal, FGF, and BMP, to replicate the stages of liver development. The sequential activation of these pathways supports early liver cell differentiation, while maturation is driven by factors such as hepatocyte growth factor (HGF) and oncostatin M (OSM) [2,3]. This precise mimicry of liver development is essential for achieving fully functional HLCs, enhancing their utility in studying liver disease progression, improving drug screening, and refining gene-editing techniques for therapeutic applications.
The advancements in iPSC-derived hepatocyte differentiation underscore the potential for combining cutting-edge stem cell technologies with molecular biology to transform research, drug discovery, and regenerative therapies for liver-related conditions.
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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