Beefy-9 media growth factor discovery kit (Qk504) Original price was: £1,675.00.£605.00Current price is: £605.00.
Beefy-9 media growth factor discovery kit (Qk504)
Original price was: £1,675.00.£605.00Current price is: £605.00.
For rapid testing of Beefy-9 against current serum-free bovine stem cell maintenance media to evaluate whether your bovine satellite cells can be expanded more efficiently. Beefy-9 is the first animal-component-free foundational medium for cultured meat and ensures food safety with no synthetic steroids, addressing regulatory concerns. It’s an open recipe, allowing for easy replication and future modifications which prevents barriers to adoption. Beefy-9 supports long-term culture over multiple passages, a critical requirement for cultured meat production, distinguishing it from prior serum-free media options.
This discovery kit allows the optimization of the published Beefy-9 recipe. Compare it with current serum-free bovine stem cell maintenance media to evaluate whether your bovine satellite cells can be expanded efficiently.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Original price was: £1,675.00.£605.00Current price is: £605.00.
1000µg will be despatched as 2 x 500µg
Fast and free shipping.
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
Bioactivity Guaranteed
>98%, by SDS-PAGE quantitative densitometry
Animal origin-free (AOF) and carrier protein-free
Expressed in E. coli
Manufactured in our Cambridge, UK laboratories
Lyophilized
- See reconstitution instructions for individual kit components
Featured applications:
Bovine and porcine stem cell expansion and maintenance
Bovine/porcine FGF-2 (154 aa) – Qk056 – 500 µg
The long form of FGF-2 used for the development of optimized serum-free culture media for species-specific bovine (cow) and porcine (pig) cultivated meat and veterinary research applications.
Bovine/porcine FGF2-G3 (154 aa) – Qk081 – 500 µg
A thermostable engineered form of bovine/porcine FGF-2. This is the 154 aa mature domain of FGF-2 (Qk056). The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3).
NRG-1 – Qk045 – 50 µg
Frequently used in the maintenance of pluripotent stem cells and has widespread use in stem cell culture media.
IGF-1 (insulin-like growth factor 1) – Qk047 – 500 µg
Used in the maintenance of pluripotent stem cells and is necessary for cell growth in the absence of insulin.
IGF-1 LR3 (insulin-like growth factor long arginine 3) – Qk041 – 500 µg
A synthetic analog of IGF-1. The substitutions include an arginine substitution and an N-terminal protein extension. Consequently, IGF-1 LR3 has improved biological potency and extended half-life.
TGF-β3 – Qk054 – 25 µg
A member of the transforming growth factor family, a family involved in regulating cell survival, proliferation and differentiation.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Find the recipe for Beefy-9 here.
The innovation of adding recombinant albumin to B8 media has yielded a ground-breaking advancement in cell culture for the growth of bovine satellite cells, resulting in the development of Beefy-9 media [1-2]. This novel medium is the first of its kind, meeting fundamental criteria to serve as a foundational medium for cultured meat production. One key feature includes the complete absence of animal-derived components, ensuring animal-component-free culture conditions. Even, vitronectin, crucial for cell adhesion to culture flasks, is produced without animal inputs. This departure from traditional serum-containing media, which often rely on animal-derived additives, distinguishes the use of Beefy-9 in the commitment to ethical and sustainable practices in cell culture [1,3-4].
Beefy-9 incorporates food-safe components, excluding any chemicals or compounds, such as synthetic steroids, that could potentially raise regulatory concerns regarding food safety. This feature enhances confidence in the safety and suitability of cultured meat products developed using Beefy-9 [1,5].
The transparency of the recipe for Beefy-9 distinguishes it from previous serum-free media options. Its fully described and defined composition, devoid of proprietary ingredients, facilitates reproducibility and allows for easy modification and optimization. Laboratories interested in advancing cultured meat research can readily adopt and build upon Beefy-9 without facing hurdles related to complex formulations or proprietary restrictions [6].
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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