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Beefy9 media discovery kit Qk504

Beefy9 media growth factor discovery kit (Qk504)

For rapid testing of Beefy-9 against current serum-free bovine stem cell maintenance media to evaluate whether your bovine satellite cells can be expanded more efficiently.

 

Beefy-9 is the first animal-component-free foundational medium for cultured meat and ensures food safety with no synthetic steroids, addressing regulatory concerns. It’s an open recipe, allowing for easy replication and future modifications which prevents barriers to adoption. Beefy-9 supports long-term culture over multiple passages, a critical requirement for cultured meat production, distinguishing it from prior serum-free media options.

This discovery kit allows the optimization of the published Beefy9 recipe. Compare it with current serum-free bovine stem cell maintenance media to evaluate whether your bovine satellite cells can be expanded efficiently.

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Orders are typically shipped same or next day (except Friday).

Buy online with secure credit card or purchase order. You can also place your order by email orders@qkine.com

Kit content

  • Bovine / porcine  FGF2 (154 aa) – Qk056 – 500 µg
    The long form of FGF-2 used for the development of optimized serum-free culture media for species-specific bovine (cow) and porcine (pig) cultivated meat and veterinary research applications.

  • Bovine /porcine FGF2-G3 (154 aa) – Qk081 – 500 µg
    A thermostable engineered form of bovine FGF-2. This is the 154 aa mature domain of FGF-2 (Qk056). The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3).

  • NRG1 – Qk045 – 50 µg
    Frequently used in the maintenance of human pluripotent stem cells and has widespread use in stem cell culture media.

  • IGF-1 (insulin-like growth factor 1)  – Qk047 – 500 µg
    Used in the maintenance of human pluripotent stem cells and is necessary for cell growth in the absence of insulin1.

  • IGF-1 LR3 (insulin-like growth factor long arginine 3) – Qk041 – 500 µg
    A synthetic analog of IGF-1. The substitutions include an Arginine substitution and an N-terminal protein extension. Consequently, IGF-1 LR3 has improved biological potency and extended half-life.

  • TGF beta 3 – Qk054 – 25 µg
    A member of the Transforming Growth Factor family, a family involved in regulating cell survival, proliferation and differentiation.1 TGF-β3 is used in human pluripotent stem cell maintenance medias, such as B8 media.

Quality features

  • Highly pure >98%, by SDS-PAGE quantitative densitometry

  • Highly bioactive – come with our Bioactivity Guarantee

  • Source: Expressed in E. coli 

  • Animal origin-free (AOF) and carrier protein-free

  • Manufactured in Cambridge, UK

  • Lyophilized

Quality assays

  • Mass spectrometry, single species with the expected mass

  • Endotoxin: <0.005 EU/μg protein (below the level of detection)

  • Recovery from stock vial: >95%

Kit description

Find the recipe for Beefy-9 here.

The innovation of adding recombinant albumin to B8 media has yielded a ground-breaking advancement in cell culture for the growth of bovine satellite cells, resulting in the development of Beefy-9 media [1-2]. This novel medium is the first of its kind, meeting fundamental criteria to serve as a foundational medium for cultured meat production. One key feature includes the complete absence of animal-derived components, ensuring animal-component-free culture conditions. Even, vitronectin, crucial for cell adhesion to culture flasks, is produced without animal inputs. This departure from traditional serum-containing media, which often rely on animal-derived additives, distinguishes the use of Beefy-9 in the commitment to ethical and sustainable practices in cell culture [1,3-4].

Beefy-9 incorporates food-safe components, excluding any chemicals or compounds, such as synthetic steroids, that could potentially raise regulatory concerns regarding food safety. This feature enhances confidence in the safety and suitability of cultured meat products developed using Beefy-9 [1,5].

The transparency of the recipe for Beefy-9 distinguishes it from previous serum-free media options. Its fully described and defined composition, devoid of proprietary ingredients, facilitates reproducibility and allows for easy modification and optimization. Laboratories interested in advancing cultured meat research can readily adopt and build upon Beefy-9 without facing hurdles related to complex formulations or proprietary restrictions [6].

[1] Stout, A. J. et al. A Beefy-R culture medium: Replacing albumin with rapeseed protein isolates. Biomaterials 296, 122092 (2023). https://doi.org/10.1016/j.biomaterials.2023.122092

[2] Kuo, H. H. et al. Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture. Stem Cell Reports 14, 256–270 (2020). doi: 10.1016/j.stemcr.2019.12.007

[3] Allen, R. E., Dodson, M. V., Luiten, L. S. & Boxhorn, L. K. A serum-free medium that supports the growth of cultured skeletal muscle satellite cells. Vitr. Cell. Dev. Biol. 21, 636–640 (1985). doi: 10.1007/BF02623296

[4] McFarland, D. C., Pesall, J. E., Norberg, J. M. & Dvoracek, M. A. Proliferation of the turkey myogenic satellite cell in a serum-free medium. Comp. Biochem. Physiol. — Part A Physiol. 99, 163–167 (1991). doi:10.1016/0300-9629(91)90252-8

[5] Kolkmann, A. M., Post, M. J., Rutjens, M. A. M., van Essen, A. L. M. & Moutsatsou, P. Serum-free media for the growth of primary bovine myoblasts. Cytotechnology 72, 111–120 (2020). doi: 10.1007/s10616-019-00361-y

[6] Mcaleer, C. W., Rumsey, J. W., Stancescu, M. & Hickman, J. J. Functional myotube formation from adult rat satellite cells in a defined serum-free system. Biotechnol. Prog. 31, 997–1003 (2015). doi: 10.1002/btpr.2063

Additional information

Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility. To ensure absolute reproducibility and optimize scientific outcomes, all our products rigorously adhere to the Nine-point Qkine Quality Commitment.

Qkine products are intended solely for research purposes and ex vivo cell manufacturing. Not for therapeutic or diagnostic use.
Discovery kits are only available on orders direct from Qkine. Distributors and resellers of Qkine products are not eligible. This product is not for resale.
No additional discount promotion can be applied to the price of the Discovery Kit. Other restrictions may apply.
If you have any questions relating to this product, please email customerservice@qkine.com.

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