Zebrafish FGF-2 is commonly used in human cell culture, but do human cells react differently to zebrafish FGF-2 when compared to human FGF-2? Bovine/porcine FGF-2 is 99% similar to human FGF-2, and mouse FGF-2 is 94% similar. Does this evolutionary divergence affect cellular response to the protein?
Novel engineered forms (like FGF2-G3), while based on human FGF-2, have more differences from human sequence than some other species. FGF2-G3 has 9 amino acid differences from the human FGF2 sequence. In comparison, only 2 of the 155 amino acids are different in bovine/porcine and human FGF-2. ‘Species neutral’ forms like this could also be compared.