Reflections on the dynamic ISSCR 2024 workshop on Standards for Human Stem Cell Use in Research including recommendations for growth factor supply chains for raw (ancillary materials) for cell therapy and ATMP manufacture.
Commercial sources of recombinant human GDF15 protein, in particular those purified from mammalian expression, are frequently contaminated with trace amounts of TGFβ and related proteins. These trace contaminants cause misleading experimental results due to the picomolar or even femtomolar EC50s of this family of cytokines. Please be cautious with your source of recombinant GDF15 protein.
Specialist bioactive growth factor protein manufacturer Qkine today announced a new partnership with StemCultures, a life sciences company dedicated to developing innovative reagents that enhance the quality and efficiency of cell growth and differentiation in culture.
Watch or listen to Catherine discuss the company’s innovations for the market, and the wider challenges for the sector on the latest episode of The Future of Protein Production podcast.
There has been an impressive drive towards innovation in stem cell protocols this year. As 2021 comes to a close, we reflect on the highlights for stem cell science over the year. It was great to see new culture media, such as B8 and “Beefy-9”, published, developments in cultivated meat science hit the news and iPSC-based therapies edge closer to the clinic. Reproducibility remains the underlying theme, and indeed requirement, for maximising the impact of these wonderful developments and at Qkine we’ll continue to do everything we can to help in 2022.
Qkine and Enantis have concluded a global licensing deal for Enantis’s patented FGF2-STAB® technology. FGF2-STAB® is a hyperstable form of FGF-2 (bFGF), a core reagent for stem cell culture. FGF2-STAB (FGF2-G3) does not degrade in culture media for enhanced weekend-free stem cell culture media and advanced chemically defined systems, such as those needed in the cultured meat industry.
There is no fundamental difference between ESGRO and recombinant mouse leukaemia inhibitor factor (mLIF) protein. Both can be used for the successful maintenance of mouse embryonic stem cell (ESC) pluripotency. 10E7 units of ESGRO is equivalent to 100 µg of pure protein. Thank you to Susanne van den Brink (postdoc in Anna Bigas lab, IMIM, Barcelona) and David Turner (University of Liverpool) for sharing their resuspension protocol to prepare ESLIF media.
We are proud to launch our Pioneering Protein range! A range of modified proteins have been carefully developed by world leading academic laboratories, licenced through Cambridge Enterprise, and then manufactured to our high purity and bioactivity standards, bringing together academic innovation with high level manufacturing purity. Folloistatin-resistant activin A (FRACTA) has been developed by our [...]
Presentation by Luana Ferrara, Senior Protein Scientist Video script Slide 1: Introduction Hello! My name is Luana Ferrara and I am a senior protein scientist at Qkine. In this short video, I’ll be presenting a comparative analysis of the purity of recombinant Activin A from different suppliers and highlighting why it's important to consider the biochemical quality of growth factors for your stem cell culture. Slide 2: The role of [...]
As a structural biologist, one will very quickly realise that protein not only needs to be pure, but it needs to be also homogeneous and the real proof of the protein’s quality is in its specific bioactivity. So,we clearly need different criteria for defining quality and that is what we do at Qkine.
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