Recombinant porcine HGF NK1 protein (Qk061-FG)

Name: Recombinant porcine HGF NK1 protein (Qk061-FG)
SKU: Qk061-FG
Availability: InStock

Recombinant porcine HGF protein is a potent, high-purity NK1 isoform of porcine hepatocyte growth factor (HGF). The porcine recombinant protein HGF differs from human HGF at several key amino acid residues that are predicted to affect bioactivity and receptor binding. Use of species-specific HGF growth factor will facilitate media optimization for cellular agriculture (cultivated meat) and veterinary applications. Also available is Qk060-FG bovine HGF (NK1). The highly scalable animal origin-free manufacture and enhanced bioactivity make this suitable for chemically-defined media and reproducible scale-up.

Qk061-FG is a 20 kDa naturally occurring isoform of HGF, animal origin-free and carrier-protein free.

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1mg will be despatched as 2 x 500µg

Available on request.
For any questions, please email orders@qkine.com

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Description

Summary

High purity recombinant porcine HGF Protein (Uniprot: A0A3Q1LSS6)
>98%, by SDS-PAGE quantitative densitometry
20 kDa
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free.
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
Resuspend in 10mM HCl at >100 µg/ml (provided with protein and free of charge), prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

Handling and Storage FAQ

Featured applications

Cellular agriculture and cultivated meat cell culture media optimization
Differentiation of iPSCs to hepatocyte-like cells
iPSC and primary cell differentiation to muscle

Alternative protein names

Hepatopoietin-A, Scatter factor, SF

Species reactivity

Porcine

Frequently used together

Recombinant bovine/porcine FGF-2 (bFGF)
Recombinant TGF beta 1 PLUS
Recombinant TGF beta 3
Recombinant thermostable FGF2-G3
Recombinant bovine HGF (NK1) protein

Bioactivity

HGF NK1 activity is determined using the Promega serum response element luciferase reporter assay (*) in HEK293T cells. EC50 = 7.0 ng/ml (338 pM). Cells are treated in triplicate with a serial dilution of HGF NK1 for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from Qk061 lot #104417. Please note: protein activity has been determined in a standardized assay using human cells. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340

Purity

Recombinant porcine HGF protein (Qk061) migrates as a single band at 18 kDa in non-reducing (NR) conditions and 20 kDa upon reduction (R). Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk061 batch #104417.

Further quality assays

Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%

Request batch-specific CoA

Download MSDS

We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Protein background

Hepatocyte growth factor (HGF) regulates cell growth, cell motility, and morphogenesis and plays a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF binds and activates the receptor tyrosine kinase, c-Met, activating PI3K/AKT, FAK, JNK, and ERK1/2 signaling [1].

HGF is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 62-kDa heavy-chain and 32-36-kDa light-chain. A disulfide bond between the heavy and light chains produces the active, heterodimeric molecule [2]. Alternative splicing of HGF produces multiple transcript variants encoding different isoforms. The NK1 isoform is the smallest naturally occurring splice variant, comprising the N-terminus and first kringle domain [3].

Bovine, porcine and human HGF are all members of the hepatocyte growth factor (HGF) family. While the sequence of porcine HGF is similar to human HGF, there are differences in structure and bioactivity. Porcine HGF is more resistant to protease digestion. Additionally, porcine HGF appears to be more potent than its counterparts when it comes to inducing muscle cell proliferation and differentiation. These findings demonstrate that porcine HGF could have important implications for cellular agriculture applications as it may provide a faster route towards creating tissues with improved regenerative qualities.

Qkine HGF (NK1) recombinant protein is produced in an animal-free microbial system that is compatible with production at scale. We are expanding our range of innovative growth factors for cellular agriculture and cultivated meat media development to support advances in the field, please reach out to cellag@qkine.com to discuss collaboration opportunities.

Background references

[1] Organ, Shawna Leslie, and Ming-Sound Tsao. ‘An Overview of the C-MET Signaling Pathway’. Therapeutic Advances in Medical Oncology, vol. 3, no. 1 Suppl, Nov. 2011, pp. S7–19. https://doi.org/10.1177/1758834011422556.

[2] Oliveira, Alexandre G., et al. ‘The Role of Hepatocyte Growth Factor (HGF) in Insulin Resistance and Diabetes’. Frontiers in Endocrinology, vol. 0, 2018. Frontiers, https://doi.org/10.3389/fendo.2018.00503.

[3] Jakubczak, John L., et al. ‘NK1, a Natural Splice Variant of Hepatocyte Growth Factor/Scatter Factor, Is a Partial Agonist In Vivo’. Molecular and Cellular Biology, vol. 18, no. 3, Mar. 1998, pp. 1275–83. doi: 10.1128/mcb.18.3.1275

Species-specificity of HGF is predicted to impact bioactivity

Porcine HGF differs from human and bovine HGF at several key amino acid residues that are predicted to affect bioactivity and receptor binding. Clustal Alignment showing sequence variation between HGF NK1 from different species. Note the lack of structural conservation at key residues, which is predicted to impact receptor binding and activation.

Sequence alignment of porcine, bovine and human HGF NK1 showing amino acid conservation

Qkine high quality food grade products are intended solely for use as food processing aids, ex vivo cell manufacturing and research use. Not for direct human consumption, therapeutic or diagnostic use.

The authorization of novel foods, including the use of growth factors as food processing aids, is regulated by regional government agencies. The use of our products as food processing aids in novel foods requires the end-user to obtain the necessary regulatory approvals.

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