Recombinant porcine TGF-β3 protein (Qk084-FG) Price range: £320.00 through £3,960.00View product
Recombinant porcine TGF-β3 protein (Qk084-FG)
Price range: £320.00 through £3,960.00
Porcine TGF-β3 protein is a member of the transforming growth factor family, a family involved in regulating cell survival, proliferation and differentiation.
Qkine is dedicated to supporting the cellular agriculture industry by manufacturing high-purity and bioactive growth factors that are species-specific to cultivated cultures. Porcine TGF-β3 is used in cultivated meat pluripotent stem cell maintenance medias, such as Beefy9 media.
Porcine high quality food grade TGF beta 3 is a protein dimer that is animal-origin free (AOF) and carrier-protein free (CF).
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £320.00 through £3,960.00
1000 µg will be dispatched as 2 x 500 µg.
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- Highly pure porcine protein (UniProt: P15203)
- 25 kDa (dimer)
High quality food grade recombinant protein
>98%, by SDS-PAGE quantitative densitometry
Animal origin-free (AOF) and carrier protein-free
Expressed in E. coli
Manufactured in the UK under a food manufacturing HACCP regime
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Maintenance and expansion of porcine iPSC, ESC and primary cells
iPSC-derived mesoderm differentiation
Cellular agriculture and cultivated meat cell culture media optimization
Frequently used together
Recombinant porcine TGF-β3 activity is determined using a TGF-β3-responsive luciferase assay. Transfected HEK293 cells are treated in triplicate with a serial dilution of porcine TGF-β3 for 6 hours. Firefly activity is measured and normalised to the control Renilla luciferase activity. EC50 = 41 pg/ml (1.6 pM).
Recombinant porcine TGF-β3 migrates at approximately 12.6 kDa (monomer) in reduced (R) and at approximately 25 kDa (dimer) in non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.05 EU/μg protein
Full raw materials traceability, allergen analysis, CoO, CoA, beta-lactam-free and animal origin-free certification available
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Transforming growth factor beta 3 (TGF-β3) is a member of the TGF-β family (and TGF-β superfamily). Like other TGF-β family members – TGF-β1 and 2 – TGF-β3 signals via receptor complexes consisting of Type I and Type II serine/threonine kinase receptors, activating intracellular SMAD transcription factors. [3]
The TGF-β family have a high degree of overlap/redundancy and regulate a variety of cellular functions, including cell survival, proliferation, and differentiation.[1] TGF beta 3 has isoform-specific roles in embryonic palate fusion4 and wound healing.[5]
In culture, TGF beta 3 is often used in porcine pluripotent cell maintenance. TGF-β3 is a component of B8 media – the low-cost weekend-free iPSC media developed in the lab of Paul Burridge, Northwestern University.[2] In the development of B8 media, Kuo et al.[2] proved that while TGF-β1 can maintain iPSCs at 2ng/ml, TGF-β3 can maintain iPSCs in as little as 0.1ng/ml, making it a cost-effective alternative to TGF-β1. This holds great promise for the development of cultivated porcine protocols and serum-free media development in the cellular agriculture industry, aiding the ultimate price-point requirements of the industry.
FAQ
What is TGF beta 3?
TGF-β3 is a growth factor involved in tissue development, wound healing, and immune regulation, crucial for iPSC and ESC proliferation and differentiation.
Where is TGF-β3 found?
TGF-β3 is found in various tissues throughout the body, including the skin, bones, blood vessels, and immune cells.
Is TGF-β3 a cytokine?
Yes, TGF-β3 is a cytokine.
What does the TGF-β3 gene do?
The TGF-β3 gene encodes the transforming growth factor beta 3 protein.
What does TGF-β3 bind to?
TGF-β3 binds to TGF-β receptors. This triggers intracellular signaling pathways that regulate gene expression, influencing cellular processes.
What is the function of the TGF-β3 receptor?
The TGF-β3 receptor serves as a cell surface receptor for the TGF-β3 ligand. It plays a crucial role in transmitting signals from the extracellular environment to the interior of the cell upon ligand binding and initiating intracellular signaling cascades that regulate gene expression.
What is the TGF-β3 pathway?
The TGF-β3 pathway is a series of intracellular signaling events triggered by the binding of TGF-β3 ligands to their cell surface receptors. Upon ligand binding, the TGF-β3 receptors undergo phosphorylation and activation, leading to the activation of downstream signaling molecules such as Smad proteins. These Smad proteins translocate into the nucleus, where they regulate the expression of target genes involved in various cellular processes such as proliferation and differentiation.
How is TGF-β3 used in cell culture?
TGF-β3 regulates cell behavior, promoting differentiation and wound healing. It is often used in tissue engineering, cellular agriculture industries for cultivated meat, and immune modulation.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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