Recombinant mouse/rat BMP-2 protein (Qk009) Price range: £230.00 through £2,900.00View product
Recombinant mouse/rat BMP-2 protein (Qk009)
Price range: £230.00 through £2,900.00
Bone morphogenetic protein 2 (BMP-2) is member of the TGF-β family and a key regulator of embryogenesis and potent differentiation factor of embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC) towards endoderm fates. BMP-2 plays roles in the differentiation of mesenchymal cells to adipocytes, epithelial cancer EMT, chondrogenesis and regulation of neuronal and glial cell development.
Qkine recombinant mouse/rat BMP-2 is a 26 kDa disulfide–linked bioactive highly pure dimer comprised of the mature domain of the BMP-2 protein, animal origin-free (AOF) and carrier protein-free.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £230.00 through £2,900.00
1000µg will be despatched as 2 x 500µg
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity BMP-2 (Uniprot: P21274)
- 26 kDa (dimer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Generation of iPSC-derived osteoblasts and chondrocytes
Growth and proliferation of chondrocytes, fibroblasts and primary cells
Neural stem cell proliferation and neuronal differentiation
Frequently used together
Alternative proteins
BMP-2 activity was determined using the BMP-2 responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of BMP-2 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 0.3 nM (7.8 ng/ml).
BMP-2 migrates as a single band at 26 kDa in non-reducing (NR) conditions and 13 kDa as a single monomeric species upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the transforming growth factor beta (TGF-β) superfamily. It plays numerous roles in the developing embryo, such as embryonic patterning along the dorso-ventral axis, organogenesis, limb bud formation, and bone and cartilage growth. BMP-2 protein is a potent differentiation factor and directs pluripotent stem cells towards extra-embryonic endoderm, mesenchymal and neural lineages, and chondrocytes [1]. Recombinant BMP-2 protein induces bone and cartilage formation in vitro and chondrogenesis in adult mesenchymal stem cells [2].
BMP-2 is synthesized as a preproprotein consisting of N-terminal signal peptide, 259 amino acid residue pro-domain and 114 residue mature domain. Proteolytic removal of the propeptide enables mature BMP-2 protein to form active disulfide-linked homodimers, and heterodimers with BMP-7.
Mature BMP-2 protein shares 100% amino acid sequence identity between human, mouse and rat. It also shares 85% amino acid sequence identity with the related protein, BMP-4, but less than 51% identity with other BMPs.
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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