Recombinant human IL-21 protein (Qk129) Price range: £320.00 through £3,960.00View product
Recombinant human IL-21 protein (Qk129)
Price range: £320.00 through £3,960.00
Interleukin 21 (IL-21) is a cytokine produced by natural killer (NK) cells and some T cells. IL-21 has broad immunological functions and can regulate NK cells, macrophages, and dendritic cells, as well as playing a role in promoting B cell and antibody responses. Recombinant IL-21 can be used in the differentiation of subsets T cells, NK cells, B cells and plasma cells.
Qkine recombinant human IL-21 is a pure and highly bioactive 15.3 kDa monomer.
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Price range: £320.00 through £3,960.00
1000 µg will be dispatched as 2 x 500 µg.
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Summary:
- High purity human protein (Uniprot: Q9HBE4)
- 15.3 kDa (monomer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Stimulation of activated T cell proliferation
Development, survival, and function of T cells
Activation of T-cells and natural killer (NK) cells
Differentiation of B cells into plasma cells
Stimulation of activated B cell proliferation
Development and formation of B cells
Recombinant IL-21 activity was determined using a STAT3 luciferase reporter assay in HEK293 cells co-transfected with the IL-21 receptors (IL-21R and IL-2Rγ). Transfected HEK293 cells were treated in triplicate with a serial dilution of IL-21 for 18 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Data from Qk129 lot #204831. EC50 = 4.17 ng/ml (0.27 nM).
Recombinant IL-21 migrates at approximately 15.3 kDa (monomer) in reduced (R) and non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk129 lot #204831.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.05 EU/μg protein
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine IL-21 was as bioactive as IL-21 from an alternative supplier. IL-21 activity was determined using a STAT3 luciferase reporter assay. Transfected HEK293 cells were treated in triplicate with a serial dilution of Qkine IL-21 (Qk129, green) or alternative IL-21 (Supplier B, black) for 18 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Data from Qk129 lot 204831.
Product background
Interleukin-21 (IL-21) is a potent immunoregulatory cytokine produced by activated Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells [1]. IL-21 expression is induced by T cell receptor signaling, and by other cytokines such as IL-1β, IL-6 and IL-27 [1]. IL-21 protein exerts its biological effects through a heterodimeric receptor composed of the IL-21-specific IL-21 receptor (IL-21R) and γ-chain (γC), which is also a receptor for IL-2, IL-4, IL-7, IL-9, and IL-15 [2]. Upon binding to its receptor IL-21 signals through the Jak-STAT signaling pathway as well as the MAPK, and PI3K pathways [1, 2].
IL-21 stimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. In combination with TGF-β1, IL-21 promotes the differentiation of human naive CD4+ T cells into Th17 cells [3]. In addition to its role in T cell biology, IL-21 also plays a critical role in B cell activation, proliferation, differentiation, apoptosis, antibody production and differentiation into plasma cells [1, 2, 4]. It is also required for the migration of dendritic cells to draining lymph nodes.
This positive effect on immune regulation has made IL-21 of interest as a therapeutic target. It may increase susceptibility of myeloid leukemia cells to CAR T and improve survival [5].
FAQ
What is interleukin-21 (IL-21)?
Interleukin-21 (IL-21) is a potent immunoregulatory cytokine.
Where is IL-21 found?
IL-21 is found primarily in the immune system and lymphoid tissues as it produced by immune cells such as activated Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells.
Is IL-21 a cytokine?
Yes, IL-21 is a γ-chain type I cytokine family, and a four-α-helix bundle pleiotropic cytokine.
What does the IL-21 gene do?
The IL21 gene is localized to human chromosome 4q26–q27 and codes for IL-21 a 131 amino acid cytokine.
What does IL-21 bind to?
IL-21 binds to a heteromer of the IL-21-specific IL-21 receptor (IL-21R) and γ-chain (γC), which is also a receptor for IL-2, IL-4, IL-7, IL-9, and IL-15.
What is the function of the IL-21 receptor?
The IL-21 receptor activates the Jak-STAT signaling pathway as well as the MAPK, and PI3K pathways.
What is the IL-21 pathway?
IL-21 binding signals through the Jak-STAT signaling pathway as well as the MAPK, and PI3K pathways.
How is IL-21 used in cell culture?
Recombinant IL-21 can be used in the study and differentiation of many immune cells including several types of T cells, B cells and plasma cells. It is also an important cytokine for cell therapy such as CAR T where is has been shown to increase effectiveness.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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