Recombinant human FGF-4 protein (Qk004)£200.00 – £1,400.00View product
Recombinant human FGF-4 protein (Qk004)
£200.00 – £1,400.00
Human fibroblast growth factor 4 (FGF-4) protein is used for the proliferation and differentiation of embryonic and induced-pluripotent and tissue (mesenchymal) stem cells and promotes neural stem cell proliferation. Recombinant FGF-4 is an important component of cardiac, intestinal and other organoid culture media.
High purity and bioactivity 14 kDa, bioactive domain of human FGF-4, animal origin-free (AOF) and carrier-protein free.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
£200.00 – £1,400.00
1000µg will be despatched as 2 x 500µg
Fast and free shipping.
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity human FGF-4 protein (residues 79-206, Uniprot: P08620)
- 14 kDa
>98%, by SDS-PAGE quantitative densitometry
Animal origin-free (AOF) and carrier protein-free
Expressed in E. coli
Manufactured in our Cambridge, UK laboratories
Lyophilized from HEPES, NaCl, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Hindgut organoids from iPSC-derived human posterior gut endoderm cells
Neural stem cell proliferation and neuronal differentiation
FGF-4 activity was determined using the firefly luciferase reporter assay in stably transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of FGF-4. Firefly luciferase activity is measured and normalized. EC50 = 306.5 pM (4.3 ng/mL). Data from Qk004 lot #104330.
Recombinant human FGF-4 protein migrates as a single band at 14 kDa in non-reducing conditions (NR) and upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk004 lot #010.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine FGF-4 bioactivity is comparable to an alternative supplier protein. Quantitative luciferase assay with Qkine FGF-4 (Qk004, green) and alternative supplier FGF-4 (Supplier B, black). Cells were treated in triplicate with a serial dilution of FGF-4 for 3 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
Technote | FGF-4 (Qk004) bioactivityProtein background
Fibroblast growth factor 4 (FGF-4) is a member of the FGF superfamily with a physiological role in the regulation of proliferation and differentiation in embryonic stem cells and tissue stem cells [1-3] .
Recombinant human FGF-4 protein promotes neural stem cell proliferation and neuronal differentiation in the postnatal brain [4], increases the proliferation rate of human adult bone-marrow derived mesenchymal stem cells [5] and supports the maintenance, proliferation and self-renewal properties of human embryonic stem cells [6].
Synergism between FGF-4 and WNT signalling acts to form hindgut organoids from iPSC-derived human posterior gut endoderm cells [7] and recombinant human FGF-4 protein is used to mimic embryonic intestinal development during directed differentiation in culture of pluripotent stem cells into intestinal organoids.
Additional resources
FAQ
What is the function of the FGF receptor?
FGFRs phosphorylate specific tyrosine residues and activate the RAS-MAPK, PI3K-AKT, PLCγ, and STAT intracellular signaling pathways.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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