Recombinant human betacellulin (Qk123)
£155.00 – £2,300.00
Out of stock – contact us support@qkine.com to be informed when stock becomes available.
Betacellulin is a member of the epidermal growth factor (EGF) family which regulates proliferation and differentiation in various stem cell types. Betacellulin stimulates proliferation and maintains pluripotency in neural and osteogenic stem cells. It can induce pancreatic β-cell differentiation in human embryonic stem cells (ESC) and differentiation of retinal progenitor cells.Â
Qkine human betacellulin is a highly pure and bioactive 9.89 kDa protein, animal origin-free (AOF), carrier-free, tag-free, and non-glycosylated to ensure its purity with exceptional lot-to-lot consistency.Â
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£155.00 – £2,300.00
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Summary:
- High purity human betacellulin (UniProt: P35070)
- 9.89 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Cellular proliferation, migration and survival
Stimulation of angiogenesis and vascular network development
Neurogenesis and neural differentiationÂ
Differentiation of retinal progenitor cells
Recombinant betacellulin activity was determined using a luciferase assay. HEK293T cells were treated in triplicate with a serial dilution of betacellulin for 3 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Data from Qk123 lot #204720. EC50 = 162.4 pg/ml.
Recombinant betacellulin migrates as a major band at approximately 15 kDa (monomer) in reduced (R) and non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein 3 µg was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk123 lot #204740.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Betacellulin was originally identified in conditioned media from mouse pancreatic-cell tumor cell line as a proliferation factor. It was discovered to be a member of the epidermal growth factor (EGF) family, binding the EGF receptor (EGF) [1, 2]. The BTC gene encodes a 178 amino acid precursor protein which is membrane anchored and cleaved to produce an 80 amino acid mature form. In addition to the EGFR, betacellulin is also a ligand for ERBB receptors, leading to activation of many intracellular signaling pathways including p38MAPK, PI3K/AKT, and JNK [1].
Initially identified as a pancreatic growth factor, betacellulin stimulates neogenesis and proliferation of pancreatic β-cells [1, 3], and pancreatic tumor cell lines [2]. It is able to covert selected non-β-cells into cells insulin producing cells, including mesenchymal stem cells (MSCs) [1, 5]. Betacellulin may also have a role in angiogenesis, it is detected in some models of retinal neovascularization and induces retinal vascular permeability and edema [1].
Betacellulin has been shown to have a regenerative role in the neural stem cell niche, stimulating proliferation and neurogenesis [5, 6]. In retinal progenitor cells (RPCs) betacellulin promotes proliferation and differentiation into retinal neurons [6]. However, in MSCs and pre-osteoblast cells it may increase proliferation but decrease osteogenic markers [7].
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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