Recombinant bovine/porcine FGF2-G3 (154 aa) protein (Qk081) Price range: £160.00 through £800.00View product
Recombinant bovine/porcine FGF2-G3 (154 aa) protein (Qk081)
Price range: £160.00 through £800.00
Recombinant bovine/porcine FGF2-G3 (154 aa) protein is a thermostable engineered form of FGF-2. Qk081 is the 154 aa mature domain of FGF-2 (Qk056). FGF2-G3 has an increased functional half-life from <10 h (wild-type) to >7 days (FGF2-G3). Bovine FGF2-G3 is used for the development of optimized serum-free culture media for species-specific bovine (cow) cultivated meat and veterinary research applications including the expansion of bovine smooth muscles cells, bovine induced pluripotent, embryonic and mesenchymal stem cells.
Recombinant FGF2-G3 is used in B8 media (Kuo et al. 2020) and Beefy-9 media (Stout et al. 2023) for weekend free, high homogeneity induced pluripotent stem cell culture. FGF2-G3 also has applications in chemically defined stem cell and organoid culture media.
Bovine/porcine thermostable FGF2-G3 (154 aa) has a molecular weight of 17.2 kDa. This protein is carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £160.00 through £800.00
1000µg will be despatched as 2 x 500µg
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity thermostable bovine/porcine FGF2-G3
- 17.2 kDa (monomer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from Tris, NaCl, CyS, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Bovine and porcine stem cell expansion and maintenance
Cellular agriculture and cultivated meat cell culture media optimization
Recombinant bovine/porcine FGF2-G3 activity was determined using the Promega serum response element luciferase reporter assay in transfected HEK293T cells. EC50 = 415 pg/ml (24 pM). Cells were treated in triplicate with a serial dilution of FGF2-G3 for 3 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk081 lot #204612.
Recombinant bovine/porcine FGF2-G3 154 aa migrates as a major band at approximately 17 kDa (monomer) in reduced (R) and non-reduced (NR) conditions. The dimeric form is also observed at approximately 34 kDa in the non-reduced condition. No contaminating protein bands are present. The purified recombinant protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk081 lot #204612.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
WT FGF-2 (Qk056) and FGF2-G3 (Qk081) were diluted in conditioned media and incubated at 37 °C. Samples were taken at 0 and 2 days. FGF-2 activity was assayed in triplicate using the Promega serum response element luciferase reporter assay in transfected HEK293T cells. Results were then normalized to the maximum response for 0 hours. Comparison between Qk056 WT FGF-2 at 0 days (EC50 0.105 ng/ml) and 2 days (no EC50). Comparison between Qk081 FGF2-G3 at 0 hours (EC50 0.128 ng/ml) and 48 hours (EC50 0.103 ng/ml).
Protein background
FGF-2 (also known as basic FGF or bFGF) is an essential growth factor for maintaining embryonic stem cell (ESC) and induced pluripotency stem cell (iPSC) pluripotency in feeder-free and chemically defined stem cell media. It is a core component of widely adopted media including mTESR [1], StemPRO [2] and E8 [3]. However, FGF-2 is inherently unstable and prone to proteolytic degradation and aggregation. This fundamental biochemical instability, and therefore low half-life in culture media (<10 h), is an important contribution to the need for frequent media changes and challenges in improving homogeneity during stem cell proliferation and subsequent differentiation.
Bovine/porcine FGF2-G3 can be a core ingredient in Beefy-9 media, established by Stout et al. in 2023. The innovation of adding recombinant albumin to B8 media has yielded a groundbreaking advancement in cell culture for the growth of bovine satellite cells, resulting in the development of Beefy-9 media. This novel medium is the first of its kind, meeting fundamental criteria to serve as a foundational medium for cultured meat production. One key feature includes the complete absence of animal-derived components, ensuring animal-component-free culture conditions. This departure from traditional serum-containing media, which often rely on animal-derived additives, distinguishes Beefy-9’s commitment to ethical and sustainable practices in cell culture [4-6].
Additional resources
FAQ
What is FGF-2?
Fibroblast growth factor 2 (FGF-2), also known as basic fibroblast growth factor (bFGF) is a growth factor and signaling protein.
What does FGF-2 do?
FGF-2 is essential for normal embryonic development. It has roles in cell survival and proliferation, angiogenesis, tumorigenesis, wound healing and tissue repair.
What does FGF-2 bind to?
FGF-2 binds to and signals though all four of the FGF receptors FGFR1-4.
What is the function of the FGF receptor?
FGFRs phosphorylate specific tyrosine residues and activate the RAS-MAPK, PI3K-AKT, PLCγ, and STAT intracellular signaling pathways.
How is FGF-2 used in stem cell culture?
FGF-2 is used to maintain the pluripotency of stem cells in culture.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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