B8 media discovery kit (Qk503) Original price was: £1,350.00.£605.00Current price is: £605.00.
B8 media discovery kit (Qk503)
Original price was: £1,350.00.£605.00Current price is: £605.00.
For current hiPSC media comparison with B8 media to see if your cells can be maintained as efficiently while giving you the weekends off. B8 hiPSC media, developed in the Burridge lab at Northwestern University, is growing in popularity thanks to its cost-effective and weekend-free cell culture credentials. B8 medium is a thermostable version of Essential 8 Medium. It is xeno-free and feeder-free medium for the growth and expansion of human pluripotent stem cells.
The B8 media Discovery kit allows the optimization of the published B8 recipe, including modified versions with higher concentrations of TGF-β1/3 (1 or 2 ng/ml), to evaluate how concentrations affect the expression of pluripotency markers. Compare B8 media with current hiPSC media to evaluate whether your cells can be maintained as efficiently with weekend-free culture.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Original price was: £1,350.00.£605.00Current price is: £605.00.
1000µg will be despatched as 2 x 500µg
Fast and free shipping.
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
>98%, by SDS-PAGE quantitative densitometry
Bioactivity Guaranteed
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized
- See reconstitution instructions for individual kit components
Featured applications:
Chemically defined media optimization
IGF-1 (insulin-like growth factor 1) – Qk047 – 500 µg
Maintains pluripotent stem cells and is necessary for cell growth in the absence of insulin.
IGF-1 LR3 (insulin-like growth factor long arginine 3) – Qk041 – 500 µg
A synthetic analog of IGF-1, substitutions include an arginine substitution and an N-terminal protein extension. Consequently, IGF-1 LR3 has improved biological potency and extended half-life.
FGF2-G3 (145 aa) – Qk052 – 100 µg
A thermostable engineered form of FGF-2. FGF2-G3 145 aa comprises the 145 aa form of FGF-2 (Qk025). The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3).
FGF2-G3 (154 aa) – Qk053 – 100 µg
A thermostable engineered form of FGF-2. FGF2-G3 154 aa is the 154 aa mature domain of FGF-2 (Qk027). The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3).
NRG-1 – Qk045 – 50 µg
Frequently used in the maintenance of pluripotent stem cells and has widespread use in stem cell culture media.
TGF-β1 PLUS – Qk010 – 25 µg
Regulates various cellular processes, including cell proliferation, growth, differentiation, motility, and apoptosis. It is an essential growth factor in many embryonic and induced pluripotent stem cell maintenance media, including the commonly used E8, StemPro, and mTeSR media.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
B8 medium is specifically designed to support the growth and expansion of human pluripotent stem cells, demonstrating its ability to uphold pluripotency across various induced pluripotent stem cell (iPSC) lines as well as embryonic stem cells (ESCs) [1-2].
B8 media is a cost-effective media designed to support cell culture without the need for daily media changes. B8 media is a thermostable version of Essential 8 (E8) Medium, which is xeno-free and feeder-free, meaning it does not contain animal-derived components and does not require feeder cells for cell growth.
B8 medium provides an optimal environment for the maintenance of pluripotency in oPSCs, making it a popular choice among researchers in the field of stem cell biology and regenerative medicine. Additionally, B8 media is often used in various experimental protocols and drug discovery studies involving iPSCs.
The activation of key signaling pathways crucial for maintaining pluripotency is facilitated by specific components within the medium. Fibroblast growth factor 2 (FGF-2) and/or neuregulin 1 (NRG-1) stimulate the PI3K/AKT/mTOR and MAPK/ERK pathways by binding to FGFR1/FGFR4 or ERBB3/ERBB4 receptors, respectively. The TGF-β signaling pathway is activated by factors such as TGF-β1, nodal, or activin A, which bind to receptors TGFBR1/2 and/or ACVR2A/2B/1B/1C. Nodal activation is less common in pluripotent medium formulations due to the presence of nodal antagonists LEFTY1/2 in iPSCs. The PI3K/AKT pathway, crucial for promoting cell survival and growth, is activated by insulin or insulin-like growth factor 1 (IGF-1), which bind to insulin receptor (INSR) and IGF1 receptor (IGF1R) respectively. These intricate signaling mechanisms underscore the precise formulation of this medium to sustain the delicate balance of pluripotency in human stem cells.
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
Receive an Amazon gift voucher when you leave us a review.
£25, $30 or €30 for reviews with an image and £10, $15 or €15 for reviews without an image