Thermostable FGF-2 discovery kit (Qk502) Original price was: £520.00.£305.00Current price is: £305.00.
Thermostable FGF-2 discovery kit (Qk502)
Original price was: £520.00.£305.00Current price is: £305.00.
For rapid testing of FGF-2 with thermostable FGF-2  protein, to determine whether the short half-life of FGF-2 is impacting cell fate. FGF-2 is commonly used in stem cell culture for induced pluripotent stem cell (iPSC) and embryonic stem cell (ESC) maintenance and induced pluripotent and mesenchymal stem cell proliferation and differentiation.
FGF-2 has a bioactive half-life of less than 10 hours. Even with daily media changes, the level of FGF2 signaling in media fluctuates substantially. FGF2-G3 is a thermostable form of FGF2 with a bioactive half-life of over 7 days, stabilized FGF-2 concentration in media and allowing weekend-free stem cell culture.
Qkine FGF-2 is available in two lengths – 145 aa or 154 aa. Recent data suggests the 145 aa form may be more bioactive at lower concentrations (Truszkowski et al, 2024).
The thermostable FGF-2 discovery kit contains 4 different forms of FGF-2 to allow comparison of FGF-2 length and thermostability on stem cell cultures.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Original price was: £520.00.£305.00Current price is: £305.00.
1000µg will be despatched as 2 x 500µg
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
>98%, by SDS-PAGE quantitative densitometry
Bioactivity Guaranteed
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized
- See reconstitution instructions for individual kit components
Featured applications:
Induced pluripotent stem cell culture and maintenance
Expansion of induced pluripotent, embryonic and mesenchymal stem cells
Chemically defined media optimization
Human FGF-2 (145 aa) – Qk025 – 50 µg
Used for induced pluripotent stem cell (iPSC) and embryonic stem cells (ESC) maintenance, and induced pluripotent and mesenchymal stem cells proliferation and differentiation.
Human FGF-2 (154 aa) – Qk027 – 50 µg
A highly bioactive, long–form of human fibroblast growth factor 2 protein. FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154 aa form of FGF-2 comprises the core structured region and N-terminal extension.
Human FGF2-G3 (145 aa) – Qk052 – 50 µg
A thermostable engineered form of human FGF-2 (bFGF). Human FGF2-G3 145 aa comprises the 145 aa form of FGF-2. The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3).
Human FGF2-G3 (154 aa) – Qk053 – 50 µg
A thermostable engineered form of human FGF-2. Human FGF2-G3 154 aa is the 154 aa mature domain of FGF-2. The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3).
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Fibroblast growth factor 2 (FGF-2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF-2 is used to support the maintenance of embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells [1-3].
FGF-2 is inherently unstable and prone to proteolytic degradation and aggregation. This fundamental biochemical instability, and therefore low half-life in culture media (<10 h), is an important contribution to the need for frequent media changes and challenges in improving homogeneity during stem cell proliferation and subsequent differentiation. To improve the stability of FGF-2 for stem cell applications, an optimal set of nine amino acid substitutions that stabilize FGF-2 were identified. The biological activity of wild-type FGF-2 is <50% after 10h incubation with conditioned media. In contrast, no reduction in FGF2-G3 biological activity is observed after >7 days incubation with conditioned media at 37oC.
Additional resources
FAQ
What is FGF-2?
Fibroblast growth factor 2 (FGF-2), also known as basic fibroblast growth factor (bFGF) is a growth factor and signaling protein.
Where is FGF-2 found?
FGF-2 is expressed in a developmental and tissue specific manner. It’s expression is tightly controlled in normal tissues and it can be detected in all major tissues.
What does FGF-2 bind to?
FGF-2 binds to and signals though all four of the FGF receptors FGFR1-4.
What is the function of the FGF receptor?
FGFRs phosphorylate specific tyrosine residues and activate the RAS-MAPK, PI3K-AKT, PLCγ, and STAT intracellular signaling pathways.
How is FGF-2 used in stem cell culture?
FGF-2 is used to maintain the pluripotency of stem cells in culture.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
Receive an Amazon gift voucher when you leave us a review.
£25, $30 or €30 for reviews with an image and £10, $15 or €15 for reviews without an image