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Thermostable FGF-2 discovery kit Qk502

Thermostable FGF-2 discovery kit (Qk502)

For rapid testing of FGF-2 with thermostable FGF-2  protein, to determine whether the short half-life of FGF-2 is impacting cell fate.

 

FGF-2 is commonly used in stem cell culture for induced pluripotent stem cell (iPSC) and embryonic stem cell (ESC) maintenance and induced pluripotent and mesenchymal stem cell proliferation and differentiation.

FGF-2 has a bioactive half-life of less than 10 hours. Even with daily media changes, the level of FGF2 signaling in media fluctuates substantially. FGF2-G3 is a thermostable form of FGF2 with a bioactive half-life of over 7 days.

FGF-2 is typically available in two lengths – 145aa or 154aa. Researchers’ rationale for using one form over the other is often based on prior use rather than definitive data.

The thermostable FGF-2 Discovery Kit contains 4 different forms of FGF-2 to determine whether there is a biological impact of using one FGF-2 length over the other in both the wild type and thermostable forms, and to determine whether consistent levels of FGF2 signaling allows for more homogeneous culture.

Fast and free worldwide shipping.
Orders are typically shipped same or next day (except Friday).

Buy online with secure credit card or purchase order. You can also place your order by email orders@qkine.com

Kit content

  • Human FGF-2 (145 aa) – Qk025 – 50 µg
    Used for induced pluripotent stem cell (iPSC) and embryonic stem cells (ESC) maintenance, and induced pluripotent and mesenchymal stem cells proliferation and differentiation.

  • Human FGF-2 (154 aa) – Qk027 – 50 µg
    A highly bioactive, long–form of human fibroblast growth factor 2 protein. FGF-2 protein is used to support the maintenance of human embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells. This 154 aa form of FGF-2 comprises the core structured region and N-terminal extension.

  • Human FGF2-G3 (145 aa) – Qk052 – 50 µg
    A thermostable engineered form of human FGF-2 (bFGF). Human FGF2-G3 145 aa comprises the 145 aa form of FGF-2. The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3).

  • Human FGF2-G3 (154 aa) – Qk053 – 50 µg
    A thermostable engineered form of human FGF-2. Human FGF2-G3 154 aa is the 154 aa mature domain of FGF-2. The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3).

Quality features

  • Highly pure >98%, by SDS-PAGE quantitative densitometry

  • Highly bioactive – come with our Bioactivity Guarantee

  • Source: Expressed in E. coli 

  • Animal origin-free (AOF) and carrier protein-free

  • Manufactured in Cambridge, UK

  • Lyophilized

Quality assays

  • Mass spectrometry, single species with the expected mass

  • Endotoxin: <0.005 EU/μg protein (below the level of detection)

  • Recovery from stock vial: >95%

Kit description

Fibroblast growth factor 2 (FGF-2) – also known as basic fibroblast growth factor (bFGF) – has a broad range of physiological roles including regulation of cell proliferation and differentiation. FGF-2 is used to support the maintenance of embryonic stem cells and proliferation and differentiation of induced pluripotent and mesenchymal stem cells [1-3].

FGF-2 is inherently unstable and prone to proteolytic degradation and aggregation. This fundamental biochemical instability, and therefore low half-life in culture media (<10 h), is an important contribution to the need for frequent media changes and challenges in improving homogeneity during stem cell proliferation and subsequent differentiation. To improve the stability of FGF-2 for stem cell applications, an optimal set of nine amino acid substitutions that stabilize FGF-2 were identified. The biological activity of wild-type FGF-2 is <50% after 10h incubation with conditioned media. In contrast, no reduction in FGF2-G3 biological activity is observed after >7 days incubation with conditioned media at 37 oC.

[1] Ludwig, T. E. et al. Derivation of human embryonic stem cells in defined conditions. Nat. Biotechnol. 24, 185–187 (2006). doi.org/10.1038/nbt1177

[2] Ludwig, T. E. et al. Feeder-independent culture of human embryonic stem cells. Nat. Methods 3, 637–646 (2006). doi.org/10.1038/nmeth902

[3] Chen, G., Gulbranson, D. R., Yu, P., Hou, Z. & Thomson, J. A. Thermal stability of fibroblast growth factor protein is a determinant factor in regulating self-renewal, differentiation, and reprogramming in human pluripotent stem cells. Stem Cells 30, 623–30 (2012). doi.org/10.1002/stem.1021

Additional information

Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility. To ensure absolute reproducibility and optimize scientific outcomes, all our products rigorously adhere to the Nine-point Qkine Quality Commitment.

Qkine products are intended solely for research purposes and ex vivo cell manufacturing. Not for therapeutic or diagnostic use.
Discovery kits are only available on orders direct from Qkine. Distributors and resellers of Qkine products are not eligible. This product is not for resale.
No additional discount promotion can be applied to the price of the Discovery Kit. Other restrictions may apply.
If you have any questions relating to this product, please email customerservice@qkine.com.

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