StemBeads® Qkine NT-3£290.00
Available on back-order
StemBeads® Qkine NT-3
£290.00
StemBeads® Qkine NT-3 is a patented growth factor supplement designed to reduce feeding times and improve the quality of NT-3 dependent cell cultures.
Please note: this product is in beta testing.
StemBeads® revolutionary controlled-release protein technology combined with Qkine high purity and bioactivity animal origin-free growth factors and cytokines stabilizes growth factor levels for increased cell culture consistency and reproducibility.
Made to order
Typically shipped within 2-3 months.
Easy world-wide ordering.
£290.00
Order fulfilled by StemCultures
Buy online with secure credit card or purchase order. For any questions, please email [email protected]
Summary:
- High purity human NT-3 protein (Uniprot: P20783)
- 27.3 kDa (dimer) 13.7 kDa (monomer)
Micro-encapsulated high purity protein
StemBeads® technology for controlled-release
Lot-to-lot consistency in bioactivity
- Product supplied in liquid form
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Product background
StemBeads® Qkine NT-3 is a patented growth factor supplement designed to culture cells with neurotrophin-3 more efficiently, with greater control and fewer medium changes. StemBeads® Qkine NT-3 are microparticles composed of an FDA approved, biodegradable polymer that is loaded with Qkine recombinant human NT-3 (Qk058, Qkine NT-3 protein is a non-covalently linked homodimer with a molecular weight of 27.3 kDa, animal origin-free (AOF), carrier-free, tag-free, and non-glycosylated to ensure its purity with exceptional lot-to-lot consistency. It is suitable for the culture of reproducible and high-quality cortical neurons and oligodendrocytes.). Under the microscope, StemBeads® will appear as small dark spheres, and with time, will break down while releasing the encapsulated protein at a controlled rate.
Please note: this product is in development and beta testing. The product is only made to order at this time (expected 6 week lead time). Data for this product is limited. Instructions for use are available upon request.
Additional resources
FAQ
Will StemBeads® interfere with my downstream application of the cells?
StemBeads® can be easily removed prior to analysis by washing 1-2 times with PBS, DMEM, F12 or any other basal medium preferred. For suspension cultures, there are a variety of strainer pore sizes to allow for separation. Cells cultured with StemBeads® have been used in a variety of downstream applications, including but not limited to: immunofluorescence, FACS, RNA/DNA extraction, nucleofection, differentiation etc.
What if I want to use a different concentration, can the protein release be adjusted?
The protein concentration can be adjusted based on the number of StemBeads® that you use. The release rate has a linear correlation with the amount of StemBeads® used. For example, 8 µl of StemBeads® per ml of media releases at a concentration of 10 ng/ml. However, if you desire half of that concentration, such as 5 ng/ml, then adjust the concentration to 4 µl of StemBeads® per ml media to achieve the concentration of 5 ng/ml.
The pH indicator in my growth medium appears very yellow when not changing media daily. Is this a problem?
No, this is not a problem or indication of cell quality. Extensive testing has been performed to access the cell quality when using StemBeads®. All phenotypic and functional assays have shown cell quality as good or better than current feeding regimens. For best assessment of cells, wash cells once prior to observation.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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