Recombinant mouse LIF protein (Qk018)£155.00 – £1,900.00View product
Recombinant mouse LIF protein (Qk018)
£155.00 – £1,900.00
Mouse LIF (murine leukemia inhibitory factor) protein maintains the pluripotency and self-renewal of mouse embryonic and induced pluripotent stem cells.
Qkine recombinant mouse LIF protein is bioactive, animal origin-free and carrier-protein free for highly reproducible results.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
£155.00 – £1,900.00
1000µg will be despatched as 2 x 500µg
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Summary:
- High purity mouse LIF protein (Uniprot: P09056)
- 20 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Propagation of mouse embryonic stem cells
Frequently used together
Alternative proteins
Mouse LIF activity was determined using the LIF-responsive firefly luciferase reporter assay in HEK293 cells co-transfected with the receptor for mouse LIF. Transfected cells were treated in triplicate with a serial dilution of LIF overnight. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from mouse LIF lot #104350. EC50 = 162 pg/ml.
Mouse LIF protein (Qk018) migrates as a single band at 20 kDa in non-reducing (NR) and upon reduction (R) with β-mercaptoethanol. Purified recombinant mouse LIF protein (3 μg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from Qk018 lot #104278.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
LIF is a pleiotrophic factor that belongs to the IL-6 superfamily of cytokines. Acting through a heterodimeric receptor of LIFR (gp190) and gp130, LIF activates a number of cellular pathways including the JAK/STAT, PI3K and MAPK pathways [1]. LIF signalling is essential for maintenance of murine pluripotency [2,3].
Human LIF can be used for the maintenance of mouse embryonic stem cells, however mouse LIF cannot bind to the human receptor, thus rendering mouse LIF inactive on human cells [4].
Customer & collaborator data
Flow cytometry analysis of Nanog-reporter positive mouse ES cells cultured using Qk018 mouse LIF vs. ESGRO. Flow cytometry was used to compare the percentage of Nanog-reporter positive mouse ES cells cultured in a chemically-defined, feeder-free iPSC culture media containing either Qk018 mouse LIF or ESGRO. Our results show that Nanog expression in mouse ES cells cultured with Qk018 mouse LIF was comparable to, if not potentially better than, ESGRO.
Experiments performed by Harry Leitch (MA MB BChir PhD MRCPCH), MRC London Institute of Medical Sciences, Imperial College.
The LIF we tested from Qkine has shown excellent quality in maintaining mouse embryonic stem cells derived from precious samples and maintaining them in a pluripotent state.
Experiments conducted by Dylan Liabeuf.
Recombinant mouse LIF protein supports mouse embryonic stem cell colony formation and has been benchmarked against LIF supplement from Supplier A in chemically-defined feeder-free iPSC culture. Cultures are dissociated to a single cell suspension and plated at very low (clonal) density in defined media containing Qk018 recombinant mouse LIF or mouse LIF supplement from another supplier (Supplier A). The number of colonies that formed was determined after 4-5 days. Data from Qk018 lot #104278 and #104279.
Our thanks to Leitch lab, MRC London Institute of Medical Sciences, Imperial College for mouse ES cell data detailed above and discussion.Mouse LIF activity was determined using the LIF-responsive firefly luciferase reporter assay in HEK293 cells co-transfected with the receptor for mouse LIF. Transfected cells were treated in triplicate with a serial dilution of LIF overnight. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. Data from mouse LIF lot #104350. EC50 162 pg/ml
Additional resources
Publications using Recombinant mouse LIF protein (Qk018)
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Distinct Molecular Trajectories Converge to Induce Naive Pluripotency
Stuart HT et al.
DOI: doi: 10.1016/j.stem.2019.07.009 -
CartoCell, a high-content pipeline for 3D image analysis, unveils cell morphology patterns in epithelia
Andrés-San Román JA, Gordillo-Vázquez C, Franco-Barranco D et al.
DOI: doi: 10.1016/j.crmeth.2023.100597 -
Chemically induced cell plasticity enables the generation of high-fidelity embryo model
Li H, Huang J, Guan W et al.
DOI: https://doi.org/10.1101/2024.06.20.598030 -
Domain Specific AI Segmentation of IMPDH2 Rod/Ring Structures in Mouse Embryonic Stem Cells
Ball STM, Hennessy MJ, Tan Y et al.
DOI: https://doi.org/10.1101/2024.10.16.617897 -
Integrative analysis of higher-order transcriptome organisation and RNA condensation principles
Klobučar T, Novljan J, Iosub IA at el.
DOI: https://doi.org/10.1101/2024.10.14.618182 -
Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition
Krammer T, Stuart HT, Gromberg E et al.
DOI: doi: 10.1016/j.devcel.2024.04.021 -
Mouse Primordial Germ Cells: In Vitro Culture and Conversion to Pluripotent Stem Cell Lines
Borkowska M, Leitch HG.
DOI: doi: 10.1007/978-1-0716-0958-3_5 -
Negative feedback on Retinoic Acid by Brachyury guides gastruloid symmetry-breaking
Hennessy MJ, Fulton T, Turner DA and Steventon B
DOI: https://doi.org/10.1101/2023.06.02.543388 -
Neural tube organoid generation: a robust and reproducible protocol from single mouse embryonic stem cells
Krammer T and Tanaka EM
DOI: https://doi.org/10.1101/2024.06.20.599764 -
Progesterone Receptor Modulates Extraembryonic Mesoderm and Cardiac progenitor Specification during Mouse Gastrulation
Drozd AM, Mariani L, Guo X, Goitea V, Menezes NA and Ferretti E.
DOI: doi: 10.3390/ijms231810307 -
Protocol for generating a 3D culture of epiblast stem cells
Rosa VS, Sato N and Shahbazi MN et al.
DOI: doi: 10.1016/j.xpro.2024.103347 -
Protocol for generating mouse morula-like cells resembling 8- to 16-cell stage embryo cells
Li H, Chang L, Huang J and Silva JCR.
DOI: doi: 10.1016/j.xpro.2024.102934 -
N2B27 media formulations influence gastruloid development
Balayo T, Lunn S, Pascual-Mas P et al.
DOI: doi.org/10.1101/2025.03.15.643474
FAQ
What is LIF?
Leukemia inhibitory factor (LIF) is an interleukin 6 class cytokine, named for its ability to induce the terminal differentiation of myeloid leukemia cells.
Where is LIF found?
In the early developing embryo, LIF is expressed in the trophectoderm.
What does LIF bind to?
LIF binds to the heterodimeric receptor LIFR (gp190)/gp130.
What is the LIFR signaling pathway?
LIF activates several cellular signaling pathways including the JAK/STAT, PI3K and MAPK pathways.
How is LIF used in stem cell culture?
LIF can be added to feeder-free stem cell cultures, it promotes self-renewal by recruiting signal transducer and activator of transcription 3 (Stat3).
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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