Recombinant human OSM protein (Qk049)£205.00 – £2,900.00View product
Recombinant human OSM protein (Qk049)
£205.00 – £2,900.00
Human OSM protein (oncostatin M) is used in the differentiation of human pluripotent stem cells into hepatocyte-like cells. OSM also plays roles in osteogenesis and neurogenesis, and is an important regulator of the hematopoietic stem cell niche in the bone marrow.
Qkine human oncostatin M protein is high purity and animal origin-free (AOF) and carrier-protein free for reproducible results.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
£205.00 – £2,900.00
1000µg will be despatched as 2 x 500µg
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Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity human OSM protein (Uniprot: P13725)
- 22 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Maturation of hepatoblasts into hepatocyte like cells
OSM activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. EC50 = 0.75 ng/ml (34 pM). Cells were treated in triplicate with a serial dilution of OSM for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk049 lot #104336. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340
OSM migrates as a single band at 20 kDa in non-reducing (NR) conditions and 22 kDa upon reduction (R). The slight band under the main band in the reduced sample is likely an artefact of the reduction protocol. No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk049 batch #104336.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Oncostatin M (OSM) is a 30 kDa secreted cytokine. OSM is a member of the Interleukin¬6 family, which includes IL¬11, CNTF and Cardiotrophin¬ 1. Human OSM is produced in cells as a 252 amino acid pre-pro-protein. The signal peptide and the C- terminal domain are proteolytically cleaved to generate the mature 195 aa form [1].
Cytokines of the IL6 family bind a common receptor subunit, gp130. Unlike other family members, OSM binds gp130 with low affinity and requires recruitment of either LIF R α or OSM R β to form the OSM receptor type I or OSM receptor type II respectively. OSM has many physiological roles and is used in several iPSC and ESC protocols. OSM has essential functions in inflammation, neuroprotection, hematopoiesis, metabolism, early development, tumorigenesis and epithelial cell growth [2].
Human OSM shares 45% aa sequence homology with mouse and rat OSM, 54% bovine and 62% porcine. There is evidence that human OSM is active on mouse cells. In stem cell applications, animal-free oncostatin M is used to promote maturation of hepatoblasts into hepatocyte-like cells for liver regenerative medicine research and drug and toxicology assay development [3-5]. Recent evidence suggests OSM can maintain naïve mouse embryonic stem cells in the absence of LIF, although the significance and general application of this observation is to be established [6]. OSM also has uses in cultured meat applications, hematopoietic stem cell differentiation and basic research.
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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