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Recombinant human FGF-9 protein (Qk039)
Human FGF-9 protein is a member of the fibroblast growth factor (FGF) family, FGF-9 is involved in many developmental processes including neural and skeletal development. Recombinant FGF-9 is used frequently in the generation of kidney organoids.
Qkine recombinant human FGF-9 is a highly pure and bioactive 23 kDa protein, animal origin-free (AOF) and carrier-protein free (CF).
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
1000µg will be despatched as 2 x 500µg
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For any questions, please email orders@qkine.com
Summary
High purity optimised human FGF-9 protein (Uniprot: P31371)
>98%, by SDS-PAGE quantitative densitometry
23 kDa (monomer)
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from HEPES/NaCl
Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20oC or -80oC
Featured applications
Kidney organoid generation
Chemically defined media
Fibroblast growth factor 9, FGF-9, Glia-activating factor, GAF, Heparin-binding growth factor 9, HBGF-9
human
species similarity:
mouse – 99%
rat – 99%
bovine – 98%
porcine – 98%
Frequently used together
Bioactivity
FGF-9 activity is determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of FGF-9 for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity. EC50 = 246.2 pM (5.7 ng/mL). Data from Qk039 lot #104295. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340
Purity
FGF-9 protein migrates as a single band at 23 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk039 lot #104295
Further quality assays
Mass spectrometry: single species with expected mass
Analytical reversed-phase: single sharp peak
Endotoxin: <0.005 EU/μg protein (below level of detection)
Recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine FGF-9 is as biologically active as a comparable alternative supplier protein
Quantitative luciferase assay with Qkine FGF-9 (Qk039, green) and alternative supplier FGF-9 (Supplier B, black). Cells were treated in triplicate with a serial dilution of FGF-9 for 3 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
Protein background
Part of the fibroblast growth factor (FGF) family, FGF-9 is a growth factor that has been implicated in many developmental processes, including in lung [1] and skeletal [2] development, sex determination [3], and neural patterning in the optic neuroepithelium [4]. Also known as glia-activating factor, FGF-9 also modulates the development and survival of neurons and glia and has been reported to play pro-survival roles (enhances cell proliferation, and alleviating cell death) in neurodegenerative diseases, such as Parkinson’s disease, Huntington’s Disease and Alzheimer’s disease [5]. FGF-9 protein is commonly used in the generation of kidney organoids [6].
FGF-9 protein binds and activates the ‘c’ splice isoform of FGF receptor 1–3 [7], and can activate a number of signalling pathways, such as ERK, STAT, PI3K and MAP kinase pathways.
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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