Recombinant human FGF-9 protein (Qk039)£155.00 – £1,900.00View product
Recombinant human FGF-9 protein (Qk039)
£155.00 – £1,900.00
Human FGF-9 protein is a member of the fibroblast growth factor (FGF) family, FGF-9 is involved in many developmental processes including neural and skeletal development. Recombinant FGF-9 is used frequently in the generation of kidney organoids.
Qkine recombinant human FGF-9 is a highly pure and bioactive 23 kDa protein, animal origin-free (AOF) and carrier-protein free (CF).
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Orders are typically shipped same or next day (except Friday).
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£155.00 – £1,900.00
500ug shipped as 2 x 250ug | 1000ug shipped as 4 x 250ug
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Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity optimized human FGF-9 protein (Uniprot: P31371)
- 23 kDa (monomer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from HEPES, NaCl
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Chemically defined media
Kidney organoid generation
FGF-9 activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of FGF-9 for 3 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. EC50 = 246.2 pM (5.7 ng/ml). Data from Qk039 lot #104295. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340
FGF-9 protein migrates as a single band at 23 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk039 lot #104295.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine FGF-9 is as biologically active as a comparable alternative supplier protein. Quantitative luciferase assay with Qkine FGF-9 (Qk039, green) and alternative supplier FGF-9 (Supplier B, black). Cells were treated in triplicate with a serial dilution of FGF-9 for 3 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
Technote | FGF-9 (Qk039) bioactivityProtein background
Part of the fibroblast growth factor (FGF) family, FGF-9 is a growth factor that has been implicated in many developmental processes, including in lung [1] and skeletal [2] development, sex determination [3], and neural patterning in the optic neuroepithelium [4]. Also known as glia-activating factor, FGF-9 also modulates the development and survival of neurons and glia and has been reported to play pro-survival roles (enhances cell proliferation, and alleviating cell death) in neurodegenerative diseases, such as Parkinson’s disease, Huntington’s Disease and Alzheimer’s disease [5]. FGF-9 protein is commonly used in the generation of kidney organoids [6].
FGF-9 protein binds and activates the ‘c’ splice isoform of FGF receptor 1–3 [7], and can activate a number of signalling pathways, such as ERK, STAT, PI3K and MAP kinase pathways.
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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