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Mesoderm differentiation kit (Qk514)

Mesoderm differentiation kit (Qk514)

For validating the ability of new or established induced pluripotent stem (iPSC) lines to differentiate into the mesoderm lineage.

The mesoderm differentiation kit is designed to validate the differentiation potential of both newly derived and established iPSC lines. This kit enables the evaluation of the ability of iPSCs to differentiate into the Mesoderm linage, one of the three primary germ layers responsible for generating muscle, bone, blood, and connective tissues.

The kit includes carefully optimized growth factors and extracellular matrix components required to efficiently guide iPSCs toward the mesoderm lineage. It serves as both an endpoint assay, confirming pluripotency and lineage commitment, and a platform for generating mesoderm-derived cells for further downstream applications.

Each kit is sufficient for differentiation of 8x 96 well plates.

Fast and free worldwide shipping.
Orders are typically shipped same or next day (except Friday).

Buy online with secure credit card or purchase order. You can also place your order by email orders@qkine.com

Kit contents

  • Human activin A – Qk001 – 25 µg
    Frequently used to maintain pluripotency in induced pluripotent and embryonic stem cell cultures. It is also used in many stem cell differentiation protocols, including endoderm lineage differentiation and further maturation into hepatocyte and pancreatic cells. 

  • Human BMP-4 – Qk038 – 25 µg
    A key regulator of embryogenesis and supports the differentiation of embryonic stem cells and induced pluripotent stem cells.

  • Human FGF2-G3 (154 aa) – Qk053 – 50 µg
    A thermostable engineered form of human FGF-2. Human FGF2-G3 154 aa is the 154 aa mature domain of FGF-2. The functional half-life has increased from <10 h (wild-type) to >7 days (FGF2-G3).

  • Human vitronectin – Qk120 – 500 µg
    Provides a defined environment that supports the maintenance of pluripotency and is suitable for feeder-free culture, expansion, differentiation, and reprogramming of stem cells.

Quality features

  • Highly pure >98%, by SDS-PAGE quantitative densitometry

  • Highly bioactive – come with our Bioactivity Guarantee

  • Source: Expressed in E. coli 

  • Animal origin-free (AOF) and carrier protein-free

  • Manufactured in Cambridge, UK

  • Lyophilized

Quality assays

  • Mass spectrometry, single species with the expected mass

  • Endotoxin: <0.005 EU/μg protein (below the level of detection)

  • Recovery from stock vial: >95%

Background

Human induced pluripotent stem cells (iPSCs) are an in vitro model that represent a pivotal breakthrough in regenerative medicine and cellular biology. iPSCs are generated by reprogramming adult somatic cells to a pluripotent state through the introduction of specific transcription factors. Reprogramming iPSCs grants these cells the ability to differentiate into any cell type of the three germ layers: ectoderm, mesoderm, and endoderm. This provides unparalleled potential for disease modeling, drug discovery, and cell-based therapies, all without the ethical concerns associated with using embryonic stem cells [1].
Differentiating iPSCs into mesoderm linage of cells is particularly significant, given the mesoderm’s role in generating a wide range of vital tissues and organs, including the heart, blood, muscles, and bones [2].

The differentiation process of iPSCs into mesodermal cells typically involves mimicking the stages of embryonic development in vitro. During embryogenesis, mesoderm formation is initiated by a series of signaling pathways that include nodal, Wnt, and bone morphogenetic protein (BMP), which are essential for mesendoderm specification and subsequent mesoderm development [3]. To mimic these conditions in a controlled laboratory environment, researchers utilize specific growth factors and small molecules to guide iPSCs through comparable developmental signals.

The successful differentiation of iPSCs into mesodermal cells is not without challenges. Due to variability within iPSC lines, differentiation efficiencies, and the potential for incomplete or mixed lineage differentiation are significant hurdles that researchers continue to address. The success can be evaluated by investigating the expression of transcription factor markers such as brachyury and MIXL1 [4].

[1] Varum, S. et al. Energy Metabolism in Human pluripotent stem cells and their differentiated counterparts. PLoS ONE. 2011;6(6):e20914. doi: 10.1371/journal.pone.0020914

[2] Loh, K. M. et al. Mapping the Pairwise Choices Leading from Pluripotency to Human Bone, Heart, and Other Mesoderm Cell Types. Cell, 2016 Jul 14;166(2):451-467. doi: 10.1016/j.cell.2016.06.011

[3] Loh, K. M. et al. Generating Cellular Diversity and Spatial Form: Wnt Signaling and the Evolution of Multicellular Animals. Developmental Cell, 2016 Sep 26;38(6):643-55. doi: 10.1016/j.devcel.2016.08.011.

[4] Lam, A. Q. et al. Rapid and Efficient Differentiation of Human Pluripotent Stem Cells into Intermediate Mesoderm That Forms Tubules Expressing Kidney Proximal Tubular Markers. Journal of the American Society of Nephrology, 2014 Jun; 25(6): 1211–1225. doi: 10.1681/ASN.2013080831

Additional information

Qkine is committed to manufacturing bioactive proteins of the highest quality to enhance scientific outcomes and improve reproducibility. To ensure absolute reproducibility and optimize scientific outcomes, all our products rigorously adhere to the Nine-point Qkine Quality Commitment.

Qkine products are intended solely for research purposes and ex vivo cell manufacturing. Not for therapeutic or diagnostic use.
Discovery kits are only available on orders direct from Qkine. Distributors and resellers of Qkine products are not eligible. This product is not for resale.
No additional discount promotion can be applied to the price of the Discovery Kit. Other restrictions may apply.
If you have any questions relating to this product, please email customerservice@qkine.com.

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