Technote | BMP-4 (Qk038)
Qkine BMP-4 has higher bioactivity than bacterial and mammalian-expressed BMP-4 from alternative suppliers
Human bone morphogenetic protein 4 (BMP-4) is a key regulator of embryogenesis and supports the differentiation of induced pluripotent stem cells (iPSC) and embryonic stem cells (ECS). BMP-4 plays a role in bone and cartilage formation, tissue repair, and organ and neuronal development. BMP-4 is part of the TGF-β superfamily and has a complex biochemical structure, expertise is required to manufacture bioactive BMP-4.
Qkine BMP-4 (Qk038) has high bioactivity, allowing lower concentrations to be used in media for cost-effective cell culture.
Qkine BMP-4 bioactivity
- Qkine animal origin-free BMP-4 (Qk038) had comparably higher bioactivity (EC50 19.4 pM) than mammalian-expressed BMP-4 (EC50 133.5 pM).
- Qkine BMP-4 bioactivity was also higher than another bacterially expressed BMP-4 (EC50 43.9 pM)
Qkine BMP-4 has higher bioactivity than alternative suppliers. Bioactivity was determined using a BMP-4-responsive firefly luciferase reporter assay in stably transfected HEK293T cells. Cells were treated with a serial dilution of BMP-4 for 6 hours in triplicate. Qkine BMP-4 (Qk038, green) has a higher bioactivity than alternative mammalian expressed (Supplier A, Peprotech 120-05ET, blue) and bacterially expressed (Supplier B, R&D Systems 314_BP/CF, grey) BMP-4. Protein concentrations were measured before use (Peprotech vial recovery 92%, R&D Systems 94%, Qkine 110%).
Qkine BMP-4 (Qk038) had higher bioactivity than BMP-4 from alternative suppliers. Qkine BMP-4 (Qk038) has the advantage of being highly pure and animal origin-free, with increased bioactivity for cost-effective reproducible stem cell culture and differentiation.
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