Technote | Activin C (Qk026)
Qkine activin C was slightly more bioactive than mammalian cell-expressed activin C from an alternative supplier
Activin C is a member of the transforming growth factor-beta (TGF-β) superfamily, a group of proteins that play key roles in regulating cell growth, survival and differentiation. Activin C activates SMAD2/3 signaling and is highly expressed in liver, testis and prostate.
Qkine recombinant activin C is the only commercially available animal origin-free activin C protein. Recombinant activin C is a bioactive 25 kDa homodimer, tag free and carrier free for improved reproducibility.
Qkine activin C bioactivity
- Qkine activin C (Qk026) was bioactive in a quantitative luciferase assay with an EC50 of 1.11 ng/ml.
- This was slightly higher than a mammalian expressed activin C protein from an alternative supplier, which had an EC50 of 2.74 ng/ml.
Qkine activin C was slightly more bioactive than mammalian cell-expressed activin C from an alternative supplier. Activin C activity was determined using a Smad reporter assay in HEK293 cells co-transfected with the activin C co-receptor ALK7. HEK293 cells were treated in triplicate with a serial dilution of Qkine activin C (Qk026, green) or R&D Systems activin C (1629-AC, Supplier A, black) for 6 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Protein concentrations were measured before use (R&D Sytems vial recovery 107 %, Qkine 98 %). Data from Qk026 lot #204801.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
All our recombinant proteins are animal-free and come with Bioactivity. Guaranteed.
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