Qkine recombinant human noggin is as biologically active as a comparable alternative supplier protein

Noggin is a bone morphogenetic protein (BMP) antagonist which regulates cell differentiation and growth during embryonic development. Noggin is used in the culture of embryonic and pluripotent stem cells and to derive neural and microglial lineages. It can also be used to generate intestinal, pancreatic, lung, and tumor-derived organoids.

Qkine noggin (Qk034) is animal origin-free, carrier-free, tag-free, and non-glycosylated to ensure high and consistent bioactivity.

• Qkine noggin (Qk034) inhibited BMP-2 (Qk007) activity in a firefly luciferase reporter assay with an EC50 of 1.97 nM.
• The inhibition was comparable with alternative noggin from Supplier B which had an EC50 of 1.39 nM.

Qkine human noggin has a comparable bioactivity from an alternative major supplier. The bioactivity of Qkine noggin (Qk034, green) and Peprotech noggin (120-10C-20UG, Supplier B, black) was determined by inhibiting BMP-2 (Qk007) activity in a BMP-2 responsive HEK293T firefly luciferase reporter assay. Cells were treated in triplicate with a serial dilution of noggin and a standard concentration of BMP-2 for 6 hours. Protein concentrations were measured before use (Peprotech vial recovery 98%, Qkine 111%). Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.

Qkine recombinant proteins are highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.