Technote | FGF-4 (Qk004)
Qkine fibroblast growth factor 4 (FGF-4) bioactivity is comparable to an alternative supplier protein
Human fibroblast growth factor 4 (FGF-4) protein is used for the proliferation and differentiation of embryonic stem cells (ESC) and induced-pluripotent stem cells (iPSC) and promotes neural stem cell (NSC) proliferation. Recombinant FGF-4 is an important component of cardiac, intestinal and other organoid culture media.
Qkine FGF-4 (Qk004) is animal origin-free, carrier protein-free and tag-free to ensure high and consistent bioactivity.
Qkine FGF-4 (Qk004) Bioactivity
- FGF-4 (Qk004) was bioactive in a quantitative luciferase assay with EC50 of 3.22 ng/ml.
- This was comparable to FGF-4 from an alternative supplier, which had an EC50 of 1.42 ng/ml.
Qkine FGF-4 bioactivity was comparable to FGF-4 from an alternative major supplier. HEK293T luciferase reporter cells were treated in triplicate with a serial dilution of Qkine FGF-4 (Qk004, green) or Peprotech FGF-4 (100-31-25UG, Supplier B, black) for 3 hours. Protein concentrations were corrected for vial recovery before use (Peprotech vial recovery 104%, Qkine 106%). Firefly luciferase activity was measured and normalized to control Renilla luciferase activity. Data from Qk004 lot #204523.
Qkine recombinant proteins have the advantage of being highly pure and animal origin-free, giving lot-to-lot consistency in bioactivity for long-term reproducible culture of stem cells and organoids.
