R-spondin-1 protein replaces conditioned media in pancreatic tumor organoid culture – data from Tuveson Laboratory, Cold Spring Harbor Lab
Comparison of pancreatic organoid growth over three passages.
P-0 indicates the initial start of the culture in full growth media supplemented with Wnt3a and R-spondin-1-conditioned media. ‘Rec. R-spondin 1’ culture conditions were created by depleting full growth media of R-spondin 1 conditioned media and supplementing it with Qk006 recombinant R-spondin 1 protein. ‘No R-spondin 1’ culture conditions were created by depleting full growth media of R-spondin 1 conditioned media but not subsequently supplementing the media with an alternative source of R-spondin 1.
No differences in organoid growth have been observed when using recombinant R-spondin-1 instead of R-spondin-1-conditioned media. Experiments have been conducted by Dennis Plenker, Ph.D. in the lab of Dr David Tuveson at Cold Spring Harbor Laboratory.
All our proteins are produced in our Cambridge, UK labs. We provide detailed quality data for each batch because we believe reliable, high-quality cytokines and growth factors are critical for successful stem cell and organoid culture.
When we test our proteins, we choose a vial at random and reconstitute as recommended to ensure we are testing as close to the protein you will receive as possible. Biochemical identity and purity is checked using SDS-PAGE, mass spectrometry and analytical reversed-phase chromatography. Bioactivity is determined using an appropriate cell-based assay. As stem cells are sensitive to endotoxin levels, we use a high resolution test to ensure endotoxin levels are at industry-leading low levels (<0.01 EU per µg protein). We also check that the correct amount of protein is recovered from the vial – it might sound basic, but if you order 100 µg, we believe you should receive 100 µg, so when you use the proteins you can rely on your calculated dilution.
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