Recombinant salmon FGF-2 (145 aa) protein (Qk102) Price range: £150.00 through £735.00View product
Recombinant salmon FGF-2 (145 aa) protein (Qk102)
Price range: £150.00 through £735.00
Recombinant salmon FGF-2 protein 145 aa (bFGF/basic FGF) for the development of optimized serum-free culture media for species-specific Atlantic salmon (Salmo salar) in cellular agriculture protocols and veterinary research applications. This shorter form of FGF-2 is used in comparative cell culture media optimization studies alongside Qk103, the 154 aa form of salmon FGF-2. FGF-2 is used extensively in the maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC) and for enhancement of proliferation in primary salmon cell culture. Receptor binding affinity and efficacy may differ depending on each species. Using a species-specific growth factor enhances receptor binding affinity, resulting in a lower concentration required in culture.
High purity 16.1 kDa salmon FGF-2 / bFGF protein, animal origin-free (AOF) and carrier-protein free (CF).
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £150.00 through £735.00
1000 µg will be dispatched as 2 x 500 µg.
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity salmon protein (Uniprot number: XP_014067501.1)
- 16.1 kDa (monomer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from Tris, NaCl, CyS, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Expansion of salmon pluripotent, embryonic and mesenchymal stem cells
Cellular agriculture and cultivated meat cell culture media optimization
Serum-free media development
Cellular proliferation, migration and survival
Alternative proteins
Recombinant salmon FGF-2 145 aa activity was determined using the Promega serum response element luciferase reporter assay (*) in transfected HEK293T cells. Cells were treated in triplicate with a serial dilution of FGF-2 for 6 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk102 lot 204618. EC50 = 2.06 ng/ml (127 pM) *Promega pGL4.33[luc2P/SRE/Hygro] #E1340
Recombinant salmon FGF-2 145 aa migrates as a major band at approximately 17 kDa (monomer) in reduced (R) and non-reduced (NR) conditions. The dimeric form is also observed at approximately 34 kDa in the non-reduced condition. No contaminating protein bands are present. The purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk102 lot #204618.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.05 EU/μg protein
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Fibroblast Growth Factor 2 (FGF-2), also known as basic fibroblast growth factor (bFGF), is a key member of the fibroblast growth factor family, playing pivotal roles in various cellular processes such as growth, differentiation, and angiogenesis [1-3]. FGF-2 is a significant growth factor in embryonic development, tissue repair and wound healing. FGF-2 (154 aa) consists of 154 amino acids and exhibits a molecular weight of around 17 kDa. It comprises multiple isoforms and is characterized by a β-trefoil fold, stabilized by disulfide bonds, essential for biological activity.
FGF-2 plays a crucial role in stem cell research development by serving as a key component in maintaining the pluripotency and self-renewal of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) [2]. FGF-2 is commonly used as a growth factor in cell culture media to maintain the pluripotent state of ESCs and iPSCs. It activates signaling pathways, including the mitogen-activated protein kinase (MAPK) pathway, which helps to sustain the expression of pluripotency-associated genes such as Oct4, Sox2, and Nanog. This prevents the differentiation of stem cells and allows for their expansion in vitro [2].
FGF-2 can be incorporated into scaffolds or hydrogels along with stem cells to promote their proliferation and differentiation within a three-dimensional environment. This holds great promise for the development of cultivated salmon protocols and serum-free media development in the cellular agriculture industry [4].
Additional resources
FAQ
What is FGF-2?
Fibroblast growth factor 2 (FGF-2), also known as basic fibroblast growth factor (bFGF) is a growth factor and signaling protein.
Where is FGF-2 found?
FGF-2 is expressed in a developmental and tissue specific manner. It’s expression is tightly controlled in normal tissues and it can be detected in all major tissues.
What does FGF-2 do?
FGF-2 is essential for normal embryonic development. It has roles in cell survival and proliferation, angiogenesis, tumorigenesis, wound healing and tissue repair.
What does FGF-2 bind to?
FGF-2 binds to and signals though all four of the FGF receptors FGFR1-4.
What is the function of the FGF receptor?
FGFRs phosphorylate specific tyrosine residues and activate the RAS-MAPK, PI3K-AKT, PLCγ, and STAT intracellular signaling pathways.
How is FGF-2 used in stem cell culture?
FGF-2 is used to maintain the pluripotency of stem cells in culture.
Why is our truncated FGF-2 145 amino acids rather than 146 amino acids?
We offer the 145 amino acid form rather than the 146 due to the E. coli processing of the N-terminal. When the 146 form (Pro143-Ser288) is expressed, the proline is either partially or completely removed. To improve expression in E. coli, we chose to start with the alanine (Ala144-Ser288) form. This modification does not affect receptor binding or bioactivity.
Other listings of the 146 amino acid form will often be a mixture of FGF-2 146 and 145, or, in some cases, only the 145 form.
What is the difference between FGF-2 145 aa and 154 aa?
Fibroblast Growth Factor 2 (FGF-2) exists in two main isoforms, distinguished by length: 145 amino acids (aa) and 154 aa. The 154 aa variant contains an additional nine amino acids at the N-terminus. While this extension is not essential for biological activity, it may influence the protein’s localization within cells.
Key differences between the isoforms:
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FGF-2 (145 aa):
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The most widely used form, supporting proliferation and survival of stem and progenitor cells.
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Commonly preferred for organoid culture due to its robust activity and well-established performance.
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FGF-2 (154 aa):
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Contains an N-terminal extension that may affect intracellular localization or signaling.
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Typically, not required for standard organoid or stem cell maintenance, unless specific signaling or localization effects are being investigated.
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What is FGF2-G3 (FGF2-STAB®)?
Recombinant FGF2-G3 (FGF2-STAB®) is a thermostable, engineered form of FGF-2 (bFGF). This modification significantly increases the protein’s functional half-life — from <10 hours for wild-type FGF-2 to >7 days for FGF2-G3 — improving stability and consistency in culture systems.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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