Recombinant rat FGF-10 protein (Qk016) Price range: £155.00 through £1,540.00View product
Recombinant rat FGF-10 protein (Qk016)
Price range: £155.00 through £1,540.00
Fibroblast growth factor 10 (FGF-10) protein promotes lung organoid formation and induces branching morphology. FGF-10 protein is used widely in organoid culture, embryonic stem cell (ESC) and induced-pluripotent stem cell (iPSC) differentiation, and for the study of epithelial to mesenchymal transition (EMT) and tumor metastasis.
Qkine recombinant rat FGF-10 is a high purity and bioactivity 17 kDa bioactive domain of FGF-10, animal origin-free (AOF) and carrier-protein free (CF).
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £155.00 through £1,540.00
1000 µg will be dispatched as 2 x 500 µg.
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity truncated FGF-10 protein (Uniprot: P70492)
- 17 kDa
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from HEPES, NaCl, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Differentiation of embryonic stem cells into gut-like structures, cardiomyocytes and hepatocytes
Epithelial to mesenchymal transition
FGF-10 activity was determined using the firefly luciferase reporter assay in stably transfected HEK293T cells. Cells are treated in triplicate with a serial dilution of FGF-10. Firefly luciferase activity is measured and normalized. EC50 = 0.36 ng/ml (21.1 pM). Data from lot #104403.
FGF-10 migrates as a single band at 17 kDa in non-reducing (NR) conditions and upon reduction (R). No contaminating protein bands are visible. Purified recombinant FGF-10 protein (7 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced conditions (NR) and stained with Coomassie Brilliant Blue R250. Data from lot #010.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial: >95%
Endotoxin: <0.05 EU/μg protein
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Qkine FGF-10 is as biologically active as a comparable alternative supplier protein. Quantitative luciferase assay with Qkine FGF-10 (Qk016, green) and alternative supplier FGF-10 (Supplier B, black). Cells were treated in triplicate with a serial dilution of FGF-10 for 4 hours. Firefly luciferase activity was measured and normalized to control Renilla luciferase activity.
Protein background
The mature form of FGF-10 protein is an approximately 20 kDa protein highly similar to FGF-7 and with a serine-rich region near its N-terminus [1]. It is secreted by mesenchymal cells and is bound and activated by extracellular FGF-BP [2]. FGF10 protein is expressed in the mesenchyme and functions through interacting with the epithelial FGF Receptor 2b (FGFR 2b) [3]. It has also been shown to interact weakly with FGFR 1b [4].
Fibroblast growth factor 10 is first active in the limb bud mesoderm where it creates and maintains FGF signaling with epithelial FGF-8, then drives a positive feedback loop accumulating mesenchyme in the growing bud, and finally induces the apical ectodermal ridge which ultimately gives rise to feet and hands [5]. Lung development is based on the same epithelial-mesenchymal FGF mediations involving FGF-10 from the foregut mesenchyme signaling to FGFR2 in the foregut epithelium [6]. Furthermore, FGF-10 protein is involved in the development of white adipose tissue, heart, liver, brain, kidney, thymus, inner ear, tongue, trachea, eye, prostate, salivary gland and mammary gland.
FGF-10 is involved in a number of different embryo and adult cell and tissue types, including mesenchymal, neuronal and epithelial cells. Recombinant FGF-10 protein drives the differentiation of embryonic stem cells (ESC) into gut-like structures, cardiomyocytes and hepatocytes [3]. It is also a potent factor in the development of organoids, increasing organoid size and branching phenotype compared to other FGFs [7].
Rat FGF-10 has 100% homology with human FGF-10.
FAQ
How is FGF-10 used in stem cell culture?
FGF-10 drives the differentiation of embryonic stem cells into gut-like structures, cardiomyocytes and hepatocytes. It allows development of large organoids with branching phenotypes.
What does FGF-10 bind to?
FGF-10 binds to and signals though FGF receptors 2b and 1b.
What is the function of FGF-10?
FGF-10 is essential for normal embryonic development, particularly in limb bud development. FGF family growth factors have roles in cell survival and proliferation, angiogenesis, tumorigenesis, wound healing and tissue repair.
Where is FGF-10 expressed?
FGF-10 is expressed in all branching morphogen organs such as the lungs, skin, ear and salivary glands.
Which cells does FGF-10 act on?
FGF-10 stimulates cell survival and proliferation on many cell types including lung and gut epithelium but has no effect on fibroblasts.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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