Recombinant human IL-8 protein (Qk119) Price range: £175.00 through £2,300.00View product
Recombinant human IL-8 protein (Qk119)
Price range: £175.00 through £2,300.00
Interleukin-8 (IL-8) is an inflammatory cytokine that acts as a chemoattractant to neutrophils. It promotes the differentiation of hematopoietic progenitors into monocytic lineages including megakaryocytes, monocytes and macrophages. IL-8 can be used for the culture and differentiation of reproducible and high-quality hematopoietic stem cells and proliferation and migration in relevant cells.
Qkine recombinant human IL-8 has a molecular weight of 8.9 kDa. This protein is animal origin-free, carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
Price range: £175.00 through £2,300.00
1000µg will be despatched as 2 x 500µg
Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity human protein (Uniprot: P10145)
- 8.9 kDa (monomer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Differentiation into monocytic lineages
Chemotaxis experiments
Differentiation of osteoclasts
Recombinant IL-8 activity was determined using an IL-8-responsive firefly luciferase reporter assay. Transfected HEK293T cells were treated in triplicate with a serial dilution of IL-8 for 3 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Data from Qk119 lot #204742. EC50 = 49 ng/ml (5.44 nM).
Recombinant IL-8 migrates as a major band at approximately 11 kDa in reduced (R) and non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein 3 µg was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk119 lot #204742.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Interleukin-8 (IL-8 or CXCL8) is a C-X-C motif family chemokine. IL-8 was originally identified through its chemotactic activity on neutrophil and named monocyte-derived neutrophil chemotactic factor (MDNCF) [1]. IL-8 is translated as 99 amino acid precursor before processing to the 72 amino acid biologically active form. In humans IL-8 has 2 receptors, CXCR1 and CXCR2, both G-protein coupled receptors [1, 4]. Binding of IL-8 to its receptors activates downstream pathways including Akt/PKB, MAPK, and PKC, regulating cell adhesion and migration.
IL-8 is an inflammatory cytokine produced by several cell types including fibroblasts, lung epithelial cells, endothelial cells, keratinocytes, T cells, neutrophils, mast cells, monocytes and macrophages [2, 4]. As well as its role in inflammation IL-8 has been shown to play a role in tumor growth and vascularization. In triple negative breast cancer, IL-8 expression is closely related to prognosis and is being investigated as a potential therapeutic target [3].
IL-8 also acts as a growth factor for human hematopoietic progenitors, promoting the growth and differentiation of cells of monocytic lineages including megakaryocytes, monocytes and macrophages [4]. Recombinant IL-8 has been shown to stimulate differentiation of human peripheral blood mononuclear cells into osteoclasts [5].
Additional resources
FAQ
What is IL-8?
IL-8 is a proinflammatory cytokine which attracts neutrophils to the site of inflammation.
Where is IL-8 found?
IL-8 is a soluble protein found in serum. IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It is also secreted by tumor cells and promotes tumor migration, invasion, angiogenesis, and metastasis.
Is IL-8 a cytokine?
Yes IL-8 is a cytokine
What does the IL-8 gene do?
IL-8 is encoded by the CXCL8, IL-8 gene expression is induced by a wide variety of agents including cytokines, growth factors, bacterial and viral products and oxidants.
What does IL-8 bind to?
IL-8 has two receptors CXCR1 and CXCR2
What is the function of the IL-8 receptor?
Both IL-8 receptors are G protein coupled and cause an increase in cGMP and activate Akt/PKB, MAPK, and PKC, regulating cell adhesion and migration.
What is the IL-8 pathway?
IL-8 binding to CXCR1 and CXCR2 activates various downstream molecules, including G proteins, small GTPases (Ras, Rac, Rho), and kinases (PI3K, PLC, PKC, AKT). This activates cell adhesion, actin polymerization, membrane protrusion, and eventually cell migration.
How is IL-8 used in cell culture?
IL-8 can be used to differentiate pluripotent cells into monocytic lineages or osteoclasts. IL-8 can also be used in chemotaxis studies.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
Receive an Amazon gift voucher when you leave us a review.
£25, $30 or €30 for reviews with an image and £10, $15 or €15 for reviews without an image