Recombinant human IL-8 protein (Qk119)£155.00 – £2,300.00View product
Recombinant human IL-8 protein (Qk119)
£155.00 – £2,300.00
Interleukin-8 (IL-8) is an inflammatory cytokine that acts as a chemoattractant to neutrophils. It promotes the differentiation of hematopoietic progenitors into monocytic lineages including megakaryocytes, monocytes and macrophages. IL-8 can be used for the culture and differentiation of reproducible and high-quality hematopoietic stem cells and proliferation and migration in relevant cells.
Qkine recombinant human IL-8 has a molecular weight of 8.9 kDa. This protein is animal origin-free, carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency.
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
£155.00 – £2,300.00
1000µg will be despatched as 2 x 500µg
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Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity human protein (Uniprot: P10145)
- 8.9 kDa (monomer)
>98%, by SDS-PAGE quantitative densitometry
Expressed in E. coli
Animal origin-free (AOF) and carrier protein-free
Manufactured in our Cambridge, UK laboratories
Lyophilized from acetonitrile, TFA
- Resuspend in 10 mM HCl (Reconstitution solution A) at >50 µg/ml, add carrier protein if desired, prepare single-use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term)
Featured applications:
Differentiation into monocytic lineages
Chemotaxis experiments
Differentiation of osteoclasts
Recombinant IL-8 activity was determined using an IL-8-responsive firefly luciferase reporter assay. Transfected HEK293T cells were treated in triplicate with a serial dilution of IL-8 for 3 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Data from Qk119 lot #204742. EC50 = 49 ng/ml (5.44 nM).
Recombinant IL-8 migrates as a major band at approximately 11 kDa in reduced (R) and non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein 3 µg was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk119 lot #204742.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Interleukin-8 (IL-8 or CXCL8) is a C-X-C motif family chemokine. IL-8 was originally identified through its chemotactic activity on neutrophil and named monocyte-derived neutrophil chemotactic factor (MDNCF) [1]. IL-8 is translated as 99 amino acid precursor before processing to the 72 amino acid biologically active form. In humans IL-8 has 2 receptors, CXCR1 and CXCR2, both G-protein coupled receptors [1, 4]. Binding of IL-8 to its receptors activates downstream pathways including Akt/PKB, MAPK, and PKC, regulating cell adhesion and migration.
IL-8 is an inflammatory cytokine produced by several cell types including fibroblasts, lung epithelial cells, endothelial cells, keratinocytes, T cells, neutrophils, mast cells, monocytes and macrophages [2, 4]. As well as its role in inflammation IL-8 has been shown to play a role in tumor growth and vascularization. In triple negative breast cancer, IL-8 expression is closely related to prognosis and is being investigated as a potential therapeutic target [3].
IL-8 also acts as a growth factor for human hematopoietic progenitors, promoting the growth and differentiation of cells of monocytic lineages including megakaryocytes, monocytes and macrophages [4]. Recombinant IL-8 has been shown to stimulate differentiation of human peripheral blood mononuclear cells into osteoclasts [5].
Additional resources
FAQ
What is IL-8?
IL-8 is a proinflammatory cytokine which attracts neutrophils to the site of inflammation.
Where is IL-8 found?
IL-8 is a soluble protein found in serum. IL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It is also secreted by tumor cells and promotes tumor migration, invasion, angiogenesis, and metastasis.
Is IL-8 a cytokine?
Yes IL-8 is a cytokine
What does the IL-8 gene do?
IL-8 is encoded by the CXCL8, IL-8 gene expression is induced by a wide variety of agents including cytokines, growth factors, bacterial and viral products and oxidants.
What does IL-8 bind to?
IL-8 has two receptors CXCR1 and CXCR2
What is the function of the IL-8 receptor?
Both IL-8 receptors are G protein coupled and cause an increase in cGMP and activate Akt/PKB, MAPK, and PKC, regulating cell adhesion and migration.
What is the IL-8 pathway?
IL-8 binding to CXCR1 and CXCR2 activates various downstream molecules, including G proteins, small GTPases (Ras, Rac, Rho), and kinases (PI3K, PLC, PKC, AKT). This activates cell adhesion, actin polymerization, membrane protrusion, and eventually cell migration.
How is IL-8 used in cell culture?
IL-8 can be used to differentiate pluripotent cells into monocytic lineages or osteoclasts. IL-8 can also be used in chemotaxis studies.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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