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Recombinant human IL-15 protein (Qk097)
Interleukin-15 (IL-15) is a pro-inflammatory cytokine which regulates immune responses. Recombinant IL-15 promotes the growth and activation of T cells and B cells and is essential in the development, survival and activation of NK cells. IL-15 can also promote conversion of monocytes into dendritic cells.
Qkine has optimized the IL-15 manufacture process to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility. Qkine IL-15 is a highly pure 12.9 kDa monomer, animal origin-free (AOF) and carrier-protein-free (CF).
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
1000µg will be despatched as 2 x 500µg
Fast and free shipping.
Buy online with secure credit card or purchase order.
For any questions, please email orders@qkine.com
Summary
High purity human IL-15 protein (Uniprot number: P40933)
>98%, by SDS-PAGE quantitative densitometry
Source: Expressed in E. coli
12.9 kDa (monomer)
Animal origin-free (AOF) and carrier protein-free
Manufactured in Cambridge, UK
Lyophilized from Acetonitrile and Trifluoroacetic acid
Resuspend in 10 mM HCl at >100 µg/ml, prepare single-use aliquots, add carrier protein if desired, and store frozen at -20°C or -80°C.
Featured applications
Development, survival, and function of T cells
Maintenance of naive and memory T cells, crucial for immune surveillance and long-term immune memory
Development and formation of B cells
Development and formation of NK cells
Hematopoietic stem cell research
Interleukin 15, MGC9721
human
species similarity:
mouse – 73%
rat – 72%
porcine -81%
bovine – 78%
Bioactivity
Recombinant IL-15 activity was determined using Promega IL-15 Bioassay Kit (JA2011). Cells were treated in triplicate with a serial dilution of IL-15 for 6 hours. Activity was measured using Bio-Glo™ Reagent and readings normalized to the maximum response. Data from Qk097 lot #204728. EC50 = 83.4 pg/ml (6.47 pM).
Purity
Recombinant IL-15 migrates as a band at approximately 12 kDa (monomer) in reduced (R) and 9 kDa in non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein 3 µg was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk097 lot #204728.
Further quality assays
Mass spectrometry, single species with the expected mass
Endotoxin: <0.005 EU/μg protein (below the level of detection)
Recovery from stock vial: >95%
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Interleukin-15 (IL-15) is a pro-inflammatory cytokine expressed in a large variety of tissues and cell types including epithelial cells, fibroblasts, keratinocytes, nerve cells, monocytes, macrophages, and dendritic cells [1]. IL-15 shares many biological properties with IL-2, despite not having sequence homology, as it interacts with components of the IL-2 receptor (IL-2R) [1, 2]. IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6 [2].
Recombinant IL-15 stimulates the proliferation and activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells [3]. IL-15 is also essential for NK cell development and can be used for the development of NK cells from hematopoietic progenitor cells [4,5].
IL-15 has been shown to play a role in several inflammatory disorders, including rheumatoid arthritis, psoriasis and pulmonary inflammatory diseases. Emerging data suggest that IL-15 may serve as a good therapeutic target, as there appears to be a beneficial effect of IL-15 neutralization in models of psoriasis and diabetes. IL-15 has also been implicated in both the development and treatment of cancers [1]. Recombinant IL-15 can also be used for the preconditioning of CAR T cells or for engineering cells to express IL-15 in vivo [6].
FAQ
IL-15 is an inflammatory cytokine.
IL-15 is a soluble protein found in blood and tissues.
IL-15 is secreted primarily by dendritic cells, monocytes, and epithelial cells.
IL-15 binds to and activates CD8+ T cells, NKT cells, γδT cells, and NK cells.
Yes, Interleukin-15 (IL-15) is a pleiotropic cytokine belonging to the common gamma-chain (γc) cytokine family, which also includes IL-2, IL-4, IL-7, IL-9, and IL-21.
The IL15 gene is localized on chromosome 4q31 and codes for interleukin-15 (IL-15), a widely expressed pro-inflammatory cytokine.
IL-15RA is expressed on the surface of activated and memory CD8+ T cells, it plays an important role in innate and adaptive immunity.
The IL-15 receptor consists of the IL-2Rβ and γc subunits in association with a unique ligand-specific subunit, IL-15Rα, IL-15Rα is expressed by lymphoid cells, DC, fibroblasts, epithelial, liver, intestine, and other cells.
IL-15 induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6.
IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It can also be used to develop monocytes into dendritic cells.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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