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Recombinant human IFN-gamma protein (Qk117)

IFN-gamma (interferon-gamma) is a cytokine crucial for immune responses, produced by T cells and natural killer cells. It activates macrophages, enhances antigen presentation, and plays a key role in inflammation.

Qkine has optimised the IFN-gamma manufacture process to produce a highly bioactive protein with excellent lot-to-lot consistency for enhanced experimental reproducibility. Qkine IFN-gamma is a highly pure 16.8 kDa protein, animal origin-free (AOF) and carrier-protein-free (CF).

Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.

1000µg will be despatched as 2 x 500µg

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For any questions, please email orders@qkine.com

Summary

  • High purity human IFN-gamma protein (UniProt number: P01579

  • >98%, by SDS-PAGE quantitative densitometry

  • Expressed in E. coli. 

  • 16.9 kDa (monomer)

  • Animal origin-free (AOF) and carrier protein-free.

  • Manufactured in our Cambridge, UK laboratories

  • Lyophilized from 20mM HEPES pH 7.5, 5% Mannitol 

  • Resuspend in water at >100 µg/ml, prepare single use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C

Featured applications

  • Hematopoietic stem cell regulation

  • Cancer immunotherapy

  • Immunoregulatory function

  • Antigen presentation enhancement

  • Involvement in infectious diseases

  • Autoimmune disease research

Immune interferon, IFN-γ

human

species similarity:
mouse – 40%
rat – 38%
porcine – 59%
bovine – 61%

Bioactivity

Human LIF Qk036 protein bioactivity lot #14293

Recombinant IFN-gamma activity was determined using a cytotoxicity assay. HT-29 cells were treated in triplicate with a serial dilution of IFN-gamma for 88.5 hours. Cell viability was measured using the CellTiter 96 AQueous Cell Proliferation Assay (Promega). Assay was performed by SBH Sciences. Data from Qk117 lot 204692. EC50 = 0.62 ng/mL (37 pM).

Purity

Human LIF Qk036 protein purity SDS-PAGE lot #14293

Recombinant IFN-gamma monomer migrates at approximately 17 kDa (monomer) in reduced (R) and non-reduced (NR) conditions. The dimeric form is also observed at approximately 34 kDa. No contaminating protein bands are present. The purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk117 lot #204692.

Further quality assays

  • Mass spectrometry: single species with expected mass

  • Endotoxin: <0.005 EU/μg protein (below level of detection)

  • Recovery from stock vial >95%

We are a company founded and run by scientists to support innovation in stem cell biology and regenerative medicine.  To enhance reliability and reproducibility in your applications, all our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.

Qkine IFN-gamma is as biologically active as a comparable alternative standard protein

Recombinant IFN-gamma activity was determined by a cytotoxicity assay using HT-29 cells. Cells were treated in duplicate with a serial dilution of Qkine IFN-gamma (Qk117, green) for 88.5 hours. Cell viability was measured using the CellTiter 96 AQueous Cell Proliferation Assay (Promega). Assay was performed by SBH Sciences using their standard (black) for comparison. Data from Qk117 lot #204692.

Protein background

IFN-gamma (interferon-gamma / IFN-γ) is a cytokine and a critical player in the immune response. It is primarily produced by T cells (particularly CD4+ and CD8+ T cells) and natural killer (NK) cells, and it plays a pivotal role in immune modulation, particularly in activating macrophages, enhancing antigen presentation (e.g., MHC I and II), and driving the Th1 immune response. IFN-gamma also has antiviral, antiproliferative, and immunoregulatory functions [1].

IFN-gamma is a non-covalent dimer with α-helical regions contributing to its overall structure, it binds to the interferon-gamma receptor (IFNGR), a transmembrane receptor composed of two subunits: IFNGR1 and IFNGR2. Upon binding to this receptor, IFN-gamma triggers the JAK-STAT signaling pathway, leading to the transcription of interferon-stimulated genes (ISGs) involved in immune regulation [1].

IFN-gamma is widely used for its immunomodulatory effects. One key application is cancer immunotherapy, which enhances the immune system’s ability to detect and eliminate tumor cells. It is also crucial in studying autoimmune diseases, as excessive production is linked to conditions like multiple sclerosis and rheumatoid arthritis [2].

IFN-gamma is commonly used in cell culture to mimic an inflammatory environment. For example, it can be applied to macrophages or other immune cells to activate them and study their response to pathogens or cancer cells. Its role in promoting antigen presentation is also exploited in vaccine development, where it boosts the efficacy of immune responses against pathogens [3].

[1] Green, Angela M., et al. “IFN-γ from CD4 T Cells Is Essential for Host Survival and Enhances CD8 T Cell Function during Mycobacterium Tuberculosis Infection.” Journal of Immunology (Baltimore, Md.: 1950), vol. 190, no. 1, Jan. 2013, pp. 270–77. https://doi.org/10.4049/jimmunol.1200061.

[2] Savan, Ram, et al. “Structural Conservation of Interferon Gamma among Vertebrates.” Cytokine & Growth Factor Reviews, vol. 20, no. 2, Apr. 2009, pp. 115–24. https://doi.org/10.1016/j.cytogfr.2009.02.006.

[3] Young, H. A., and J. H. Bream. “IFN-Gamma: Recent Advances in Understanding Regulation of Expression, Biological Functions, and Clinical Applications.” Current Topics in Microbiology and Immunology, vol. 316, 2007, pp. 97–117. https://doi.org/10.1007/978-3-540-71329-6_6.

FAQ

IFN-gamma is a cytokine crucial for immune responses, produced by T cells and natural killer cells. It activates macrophages, enhances antigen presentation, and plays a key role in inflammation.

IFN-gamma is primarily produced by activated T cells (especially CD4+ Th1 cells and CD8+ cytotoxic T cells) and natural killer (NK) cells. It is found in tissues and the bloodstream, particularly at sites of infection or inflammation where immune responses are active.

Yes, IFN-gamma is a cytokine, playing a vital role in immune regulation, particularly in promoting inflammation.

The IFN-gamma gene (IFNG) encodes the IFN-gamma protein, a cytokine that regulates immune responses.

IFN-gamma binds to the IFN-gamma receptor (IFNGR), a heterodimer composed of two subunits: IFNGR1 and IFNGR2.

The IGF-2 receptor (IGF-2R), also known as the mannose-6-phosphate receptor (M6P receptor), functions in regulating IGF-2 levels by binding to and facilitating its degradation. Unlike the IGF-1 receptor, IGF-2R does not promote cell growth or survival. Instead, it acts as a scavenger receptor, helping to control IGF-2 activity and prevent excessive cell proliferation.

The IFN-gamma receptor (IFNGR) mediates the effects of IFN-gamma by activating intracellular signaling pathways, particularly JAK-STAT. It enhances gene expression related to immune responses, activates macrophages, influences T cell differentiation toward a Th1 response, and boosts antiviral and antitumor activities, playing a crucial role in immunity

The IFN-gamma pathway involves IFN-gamma binding to its receptor, activating JAKs, and phosphorylating STAT1. This leads to STAT1 dimerization and translocation to the nucleus, where it regulates gene expression related to antigen presentation, inflammation, and immune cell activation.

IFN-gamma is used to activate immune cells, induce differentiation, modulate immune responses, and influence stem cell behavior. IFN-gamma helps study infection models, cancer research, and cytokine secretion by enhancing antigen presentation.

Our products are for research use only and not for diagnostic or therapeutic use.  Products are not for resale.

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