Recombinant human FGF-8b protein (Qk057-CTG) Price range: £3,250.00 through £5,060.00View product
Recombinant human FGF-8b protein (Qk057-CTG)
Price range: £3,250.00 through £5,060.00
Fibroblast growth factor 8b (FGF-8b) is a member of the FGF family involved in the regulation of embryogenesis, cellular proliferation, differentiation, and migration. FGF-8b is commonly used for the differentiation of induced pluripotent stem cells into neural cell types and brain organoid cultures. FGF-8b is a spliced form of FGF-8, a heparin-binding protein that targets mammary and non-mammary cells expressing the FGF receptors.
Qkine FGF-8a is a 22.5 kDa highly pure, bioactive recombinant protein produced in an animal origin-free expression system. This protein is carrier-free, tag-free and non-glycosylated to ensure a pure, homogenous protein with exceptional lot-to-lot consistency, suitable for cell therapy development.
Qkine cell therapy grade high purity animal origin-free proteins are manufactured as GMP grade equivalents in an ISO 9001:2015-certified facility, under ISO 20399:2022 standards with GMP compliance, defined quality criteria and documentation.
This product is available to order now and will be in stock soon, please contact us for the estimated lead time customerservice@qkine.com
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Orders are typically shipped same or next day (except Friday).
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Price range: £3,250.00 through £5,060.00
1000µg will be despatched as 2 x 500µg
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Summary:
- High purity human FGF-8b protein (Uniprot: P55075)
- 22.5 kDa
Certified animal origin-free (AOF)
Cell and gene therapy grade
Manufactured in our ISO 9001:2015 facility in Cambridge, UK
Lot-to-lot consistency in bioactivity
Full traceability and documentation
Carrier protein-free, tag free
Expressed in E. coli
Lyophilized from Tris, NaCl, CyS, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Pluripotent stem cell therapy
Neural stem cell (NSC) therapy
Regenerative stem cell therapy
Alternative proteins
FGF-8b activity was determined using the Promega serum response element luciferase reporter assay (*) in HEK293T cells. EC50 = 4.1 ng/ml (0.18 nM). Cells were treated in triplicate with a serial dilution of FGF-8b for 3 hours. Firefly luciferase activity was measured and normalized to the control Renilla luciferase activity. Data from Qk057 lot #104458. *Promega pGL4.33[luc2P/SRE/Hygro] #E1340
FGF-8b (Qk057) migrates as a single band at 22.5 kDa in non-reducing (NR) conditions and upon reduction. Purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptothanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk057 batch #104458.
Further quality assays
Mass spectrometry: single species with expected mass
Purity >98%, by SDS-PAGE quantitative densitometry
Recovery from stock vial: >95%
Sterility tested and mycoplasma negative
Endotoxin: <0.005 EU/μg protein (below level of detection)
Residual host cell protein <10 ng/µg
Residual host cell DNA <10 ng/μg
N-terminal sequence analysis
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
FGF-8b is a heparin-binding protein and an isoform of FGF-8 belonging to a family of fibroblast growth factors (FGF) [1]. It was originally discovered as an essential growth factor for the androgen-dependent growth of mouse mammary carcinoma cells [2]. In mouse, there are eight sliced protein isoforms of FGF8 (a-h) whereas in humans, there are four alternate spliced protein isoforms namely FGF-8a, FGF-8b, FGF-8e and FGF-8f. These four FGF8 isoforms (a, b, e and f) are highly conserved between humans and mice. Human and murine FGF-8a and FGF-8b show 100% homology [3] whereas there is a 98% identity with human and murine FGF8e and FGF8f [4].
Human FGF-8b, a monomeric protein has a molecular weight of 22.5kDa with 194 amino acid (aa) residues covering the signal sequence domain, N-terminal domain, FGF domain and proline-rich C terminal sequence. Its three-dimensional structure is composed of 12 beta strands arranged in two beta-sheet and short alpha-helices. The protein contains a conserved heparin-binding domain that is essential for its biological activity.
FGF-8, including the spliced forms, work by binding the FGF receptors (FGFR) to activate the Ras/MAPK signalling pathway, a key pathway that contributes to several cellular processes. In general, the FGF family is involved in broad cellular and biological processes including cell proliferation, differentiation, survival and apoptosis [5-8].
Functionally, FGF-8b has been shown to play a major role during prenatal development. It is widely expressed during embryogenesis and is a key player in epithelial-mesenchymal transitions [9]. During gastrulation, it contributes to the organization and induction role and regulates the patterning of organs in the embryos. These organs include the brain, eye, ear, limb and the heart [10-12].
Although, FGF-8 isoforms work in a coordinated and concerted manner, findings have suggested that they also have distinct key roles. FGF-8b has been shown to have the strongest affinity for the receptor and oncogenic capacity. A study using transgenic mice showed FGF-8a expands the midbrain while FGF-8b showed a transformational activity by transforming the midbrain into the cerebellum [13].
FGF-8b and other neurotrophins have been shown to promote neural regeneration. More specifically, FGF-8b has been shown to promote neurite outgrowth in mammalian spiral ganglion neurons (SGN) in vitro [14]. FGF-8b, in combination with Shh, a neurotrophic factor, and transcription factors Ascl1 and Nurr1, has been used to generate induced neuronal cells (pan-neuronal and dopaminergic (DA) neurons) by reprogramming embryonic mouse fibroblasts [15]. Additionally, FGF-8b has been used to generate DA neurons from stem cells, including induced pluripotent stem cells (iPSCs) and dental pulp stem cells [16-17]. More recently, FGF-8b has been used to generate ventral midbrain organoids derived from iPSCs to provide a robust 3D in vitro platform that is suitable for comprehensive DA neuronal studies [18].
Whilst there is a limited expression of FGF-8 and its isoforms in the normal adult, increasing studies have shown the presence of FGF-8 in adult tissues and cells including the reproductive tract, peripheral leukocytes and hematopoietic cells [19-20]. Further functional studies are required to fully delineate the role of FGF-8 and its isoforms in the normal adult.
Additional resources
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
For use in manufacturing of cellular or gene therapy products. Not intended for in vivo applications.
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