Recombinant chicken/duck FGF-2 (145 aa) protein (Qk030)£120.00 – £670.00View product
Recombinant chicken/duck FGF-2 (145 aa) protein (Qk030)
£120.00 – £670.00
Recombinant chicken/duck FGF-2 protein 145 aa (bFGF/basic FGF) for the development of species-specific avian cellular agriculture protocols and veterinary research applications. Used in comparative cell culture media optimization studies for cellular agriculture process development. FGF-2 is used extensively in the maintenance and proliferation of induced pluripotent (iPSC) and embryonic stem cells (ESC) and for enhancement of proliferation in primary cell culture.Â
Qkine chicken/duck FGF-2 protein is a high purity 16 kDa protein, animal origin-free (AOF), tag free and carrier-protein free (CF).Â
In stock
Orders are typically shipped same or next day (except Friday).
Easy world-wide ordering, direct or through our distributors.
£120.00 – £670.00
1000µg will be despatched as 2 x 500µg
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Buy online with secure credit card or purchase order. For any questions, please email orders@qkine.com
Summary:
- High purity chicken/duck protein (UniProt: P48800/ A0A6J3CX03)
- 16 kDa (monomer)
>98%, by SDS-PAGE quantitative densitometry
Animal origin-free (AOF) and carrier protein-free
Expressed in E. coli
Manufactured in our Cambridge, UK laboratories
Lyophilized from Tris, NaCl, CyS, mannitol
- Resuspend in sterile-filtered water at >50 µg/ml, add carrier protein if desired, prepare single use aliquots and store frozen at -20 °C (short-term) or -80 °C (long-term).
Featured applications:
Chicken and duck primary and stem cell cultureÂ
Cellular agriculture process development
Cellular agriculture and cultivated meat cell culture media optimization
Frequently used together
Alternative proteins
Recombinant chicken/duck FGF-2 145 aa activity was determined using FGF-2 responsive firefly luciferase reporter assay. Transfected HEK293T cells were treated in triplicate with a serial dilution of chicken/duck FGF-2 145 aa for 3 hours. Firefly activity was measured and normalized to the control Renilla luciferase activity. Data from Qk037 lot 204733. EC50 = 0.256 ng/ml.
Recombinant chicken/duck FGF-2 145 aa migrates as a major band at approximately 16 kDa (monomer) in reduced (R) and non-reduced (NR) conditions. No contaminating protein bands are present. The purified recombinant protein (3 µg) was resolved using 15% w/v SDS-PAGE in reduced (+β-mercaptoethanol, R) and non-reduced (NR) conditions and stained with Coomassie Brilliant Blue R250. Data from Qk030 lot #204733.
Further quality assays
Mass spectrometry: single species with expected mass
Recovery from stock vial:Â >95%
Endotoxin: <0.005 EU/μg protein (below level of detection)
We are a company founded and run by scientists to provide a service and support innovation in stem cell biology and regenerative medicine. All our products are exceptionally high purity, with complete characterisation and bioactivity analysis on every lot.
Protein background
Fibroblast growth factors (FGFs) are a family of growth factors which regulate a wide range of essential biological functions including cell proliferation and survival, migration and differentiation [1]. The human FGF family has 18-22 members grouped into 6-7 subfamilies based on sequence homology and phylogeny [2]. The FGF family of growth factors have critical roles during both vertebrate and invertebrate embryonic development, in adult cells they modulate tissue maintenance, wound healing and angiogenesis [1,2].
The FGF ligands bind to 4 receptors (FGFR1-4), transmembrane receptors with intracellular tyrosine kinase activity [2, 3]. Once activated FGFRs recruit Src homology-2 (SH2) or phosphotyrosine binding (PTB) domain-containing signaling proteins leading to activation of intracellular signaling pathways [1]. The main signaling pathways activated by FGF binding are the RAS/MAP kinase pathway, PI3 kinase/Akt pathway, and PLCγ pathways [1-3].
Fibroblast growth factor 2 (FGF-2) also known as basic fibroblast growth factor (bFGF) has a broad range of physiological roles including regulation of cell growth, survival and proliferation. FGF-2 is one of the FGFs which binds to and signals through all four of the FGFRs [4].
FGF-2 is an essential growth factor for the maintenance of pluripotency in human embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs) [4] and mesenchymal stem cells (MSCs) [5]. FGF-2 preserves pluripotency through interaction with PI3 kinase/Akt, ERK1/2, JAK/STAT and PLCγ pathways by activation of Activin A. In feeder culture systems FGF-2 stimulates production of essential growth factors and cytokines from the feeder layer [5].
Additional resources
FAQ
What is FGF-2?
Fibroblast growth factor 2 (FGF-2), also known as basic fibroblast growth factor (bFGF) is a growth factor and signaling protein.
Where is FGF-2 found?
FGF-2 is expressed in a developmental and tissue specific manner. It’s expression is tightly controlled in normal tissues and it can be detected in all major tissues.
What does FGF-2 do?
FGF-2 is essential for normal embryonic development. It has roles in cell survival and proliferation, angiogenesis, tumorigenesis, wound healing and tissue repair.
What does FGF-2 bind to?
FGF-2 binds to and signals though all four of the FGF receptors FGFR1-4.
How is FGF-2 used in stem cell culture?
FGF-2 is used to maintain the pluripotency of stem cells in culture.
What is the function of the FGF receptor?
FGFRs phosphorylate specific tyrosine residues and activate the RAS-MAPK, PI3K-AKT, PLCγ, and STAT intracellular signaling pathways.
Our products are for research use only and not for diagnostic or therapeutic use. Products are not for resale.
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